关键词: 梨属, 自交不亲和性, SFBB-&gamma, 基因

Abstract:

By using PCRbased molecular method，four new pear SFBB^-γ genes were isolated from 8 pear cultivars known as Sgenotypes. Length of PCR products from eight pear cultivars was around 1 200 bp. Sequencing the specific PCR fragments revealed four new SFBB^-γ genes that were respectively named as SFBB^16-γ（EU422956）, SFBB^-17-γ（EU422957）, SFBB²⁸-γ（EU422960）and SFBB^35-γ（EU422958）. As for different SFBB^-γ genes, variation in amino acid was higher in Fbox region and variable region 1, and lower in variable region 2 to 4 Phylogenetic analysis showed that there was a high level of difference between Japanese pear（Pyrus pyrifolia） SFBB^-γ genes and Xinjiang pear（P. sinkiangensis/i>） SFBB^-γ genes, as well as Japanese pear SFBB^-γ genes and Chinese white pear（P. bretschneideri） SFBB^γ genes. The results might reflect their genetic relationships among the species.

Key words: Pyrus, selfincompatibility, SFBB^-&gamma, gene