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林业科学 ›› 2009, Vol. 12 ›› Issue (12): 154-158.doi: 10.11707/j.1001-7488.20091227

• 论文 • 上一篇    下一篇

红平菇木质素降解酶系统漆酶、锰过氧化物酶及木质素过氧化物酶的检测池

池玉杰 闫洪波   

  1. 东北林业大学林学院 哈尔滨 150040
  • 收稿日期:2009-05-29 修回日期:1900-01-01 出版日期:2009-12-25 发布日期:2009-12-25
  • 基金资助:
     

Detection on Laccase, Manganese Peroxidase and Lignin Peroxidase in Ligninolytic Enzymes of Pleurotus djamor

Chi Yujie,Yan Hongbo   

  1. College of Forestry,Northeast Forestry University Harbin 150040
  • Received:2009-05-29 Revised:1900-01-01 Online:2009-12-25 Published:2009-12-25
  • Supported by:
     

摘要:  

关键词: 红平菇, 木质素降解酶系统, 锰过氧化物酶, 漆酶, 酶活检测

Abstract:

White rot fungus Pleurotus djamor H1 was stilly cultured at 28 ℃ under 4 different kinds of LNAS culture solution, the extracellular enzyme solutions were sampled at different interval, O. D. values of the solutions, representing manganese peroxidase (MnP), laccase and lignin peroxidase (LiP) activities, were measured spectrophotometrically by monitoring the oxidation of 2, 6-DMP at 470 nm, ABTS at 420 nm and veratryl alcohol(VA) at 310 nm, the lignin-degrading enzymes of the fungus and the relationships between enzyme activities and medium composition and substrates were gained. Results indicated that Pleurotus djamor could produce MnP and laccase simultaneously, but no Lip. Substrates wood sawdust and 2, 6-DMP could significantly enhanced MnP and laccase activities, the biggest Mnp and laccase activity were 5 U·L-1.and 38 U·L-1when no substrates were added, and 69 U·L-1 and 205 U·L-1 when substrates added. The paper offered a basic ligninolytic enzymology of Pleurotus djamor for future utilization of the enzymes and further study on MnP and laccase mechanism of the fungus.

Key words: Pleurotus djamor, ligninolytic enzymes, manganese peroxidase, laccase, enzyme activity detection

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