欢迎访问林业科学,今天是

林业科学 ›› 2009, Vol. 12 ›› Issue (5): 164-168.doi: 10.11707/j.1001-7488.20090526

• 论文 • 上一篇    下一篇

陕西省31株木本植物炭疽菌的rDNA-ITS区序列分析*

张 振 余仲东 唐 明 侯 璐   

  1. (西北农林科技大学林学院 杨凌 712100)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-05-25 发布日期:2009-05-25
  • 通讯作者: 余仲东

ITS-rDNA Sequence Analysis of Colletotrichum in Woody Plants in Shaanxi Province

Zhang Zhen Yu Zhongdong Tang Ming Hou Lu   

  1. (College of Forestry, Northwest Agricultural and Forestry University Yangling 712100)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-25 Published:2009-05-25

关键词: 炭疽菌属, rDNA-ITS, 多样性, 系统进化

Abstract:

Thirty one isolates of Colletotrichumspp. from different woody plants in Shaanxi province were collected and identified by morphological characteristics. Genetic diversity and phylogeny among the 31 isolates were investigated by sequence analysis based on the rDNA-ITS sequence variation, and compared with 11 relevent sequences from GenBank. The results showed that there was rich genetic diversity in Colletotrichum species; and the sequences diversity index was 01957. All the Colletotrichum isolates were clustered into three groups at the bootstrap value of 94%. The Colletotrichum gloeosporioides isolates were clustered into the G1、G2 and G3, respectively. Some isolates of this species exhibited extensive genetic variability compared with the other specie isolates. There was also genetic difference among the isolates collected from the same host tree. It is reasonable to use complex species (Colletotrichum gloeosporioides) to refer to the woody Colletotrichum pathogens at present.

Key words: Colletotrichum, rDNA-ITS, genetic diversity, phylogeny