关键词: 倍蚜, 随机扩增多态性DNA, 亲缘关系, 遗传距离, 聚类分析

Abstract:

The RAPD technique was used to determine phylogenetic relationships of Chinese gallnut aphids and genetic differentiation in four populations of Schlechtendalia chinensis, a major Chinese gallnut aphid. 13 primers and 9 primers were selected from 40 primers and used to amplify their genome DNA. The genetic distances between genera, species and subspecies are 0.482 8 ± 0.170 8,0.252 0 ±0.178 0,0.147 2 ± 0.076 4, respectively. Dendrogram based on Nei’s genetic distance displayed the phylogenetic relationships of different taxa and almost coincided with morphological taxonomy. Our result suggested that there is a distinct difference between the Nurudea and other three genera. It may be implied that the Nurudea was the first differentiation group among Chinese gall aphids. The genetic distance among 4 populations of S. chinensis is 0.075 9± 0.030 2. A certain extent genetic variation among the populations had emerged and it maybe caused by the geographical isolation.

Key words: Chinese gallnut aphids, RAPD, phylogenetic relationship, Nei&rsquo, s genetic distance, cluster analysis