杜仲叶酸性多糖提取分离及含量测定

doi:10.11707/j.1001-7488.20061011

林业科学 ›› 2006, Vol. 42 ›› Issue (10): 59-64.doi: 10.11707/j.1001-7488.20061011

杜仲叶酸性多糖提取分离及含量测定

董娟娥¹ 梁宗锁¹ 靳爱仙² 余震宇¹ 成密红¹

1.西北农林科技大学,杨凌712100；2.国家林业局调查规划设计院,北京100714

收稿日期:2006-03-29 修回日期:1900-01-01 出版日期:2006-10-25 发布日期:2006-10-25

The Extraction and Content Measurement of Acidic Polysaccharides from Eucommia ulmoides Leaves

Dong Juan'e¹,Liang Zongsuo¹,Jin Aixian²,Yu Zhenyu¹,Cheng Mihong¹

1. Northwest Sci-Tech University of Agricuture and Forestry Yangling 712100;2. Academy of Forest Inventory and Planning,State Forestry Administration Beijing 100714

Received:2006-03-29 Revised:1900-01-01 Online:2006-10-25 Published:2006-10-25

摘要/Abstract

摘要：

研究杜仲叶酸性多糖的提取分离工艺及其含量测定的简便方法。结果表明,以提取过药用有效成分后的杜仲叶为原料提取酸性多糖的较佳工艺条件是1.0%的碱水在100℃下提取2次,每次2h;提取液经大孔吸附树脂处理,多次醇沉等步骤分离的酸性多糖含量可达到41.46%。采用DNS法测定杜仲叶酸性多糖含量时水解时间控制在15～20min,显色反应为10min,检测波长为492nm;在试验条件下,葡萄糖浓度在0.20～0.60mg·mL^-1范围内显色灵敏、稳定,线性关系良好;用该法测定杜仲叶渣酸性多糖含量时加样回收率为98.30%,RSD为1.76%;显色溶液在2h内吸光度值比较稳定,能够完全满足测定工作要求,可以作为实验室测定多糖含量的简便、快捷、有效的方法。

关键词: 杜仲叶, 酸性多糖, 提取分离, 含量测定

Abstract:

The extraction techniques and a simple measurement method for the acidic polysaccharides from Eucommia ulmoides leaves were studied. The results were as follows:The residue of E.ulmoides leaves after extracting bioactive constituents could be used to extract acidic polysaccharides,which was the multilevel exploitation and reuse of E.ulmoides. The optimum extraction condition was 1.0% ammonia solvent,at the temperature of 100 ℃,2 times and 2 h per time. The acidic polysaccharides could be purified with resin. To measure the contents of acidic polysaccharides with DNS method,the hydrolyzed time should be within 15～20 min,the optimum coloration time was 10 min,the maximum absorption wavelength was 492 nm,and the recovery was 98.30%,RSD,1.76%.

Key words: Eucommia ulmoides leave, acidic polysaccharides, extraction and purification, content measurement

董娟娥梁宗锁靳爱仙余震宇成密红. 杜仲叶酸性多糖提取分离及含量测定[J]. 林业科学, 2006, 42(10): 59-64.

Dong Juan'e;Liang Zongsuo;Jin Aixian;Yu Zhenyu;Cheng Mihong. The Extraction and Content Measurement of Acidic Polysaccharides from Eucommia ulmoides Leaves[J]. Scientia Silvae Sinicae, 2006, 42(10): 59-64.

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杜仲叶酸性多糖提取分离及含量测定

The Extraction and Content Measurement of Acidic Polysaccharides from Eucommia ulmoides Leaves

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