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林业科学 ›› 2006, Vol. 42 ›› Issue (6): 141-144.doi: 10.11707/j.1001-7488.20060623

• 研究简报 • 上一篇    

不同寄主上的蔗扁蛾ITS分析

沈杰1 高其康2 沈幼莲3 楼兵干2   

  1. 1浙江省森林植物防治检疫站,杭州310004;2.浙江大学,杭州310029;3.浙江省慈溪市森林植物检疫站,慈溪315300
  • 收稿日期:2005-05-09 修回日期:1900-01-01 出版日期:2006-06-25 发布日期:2006-06-25

ITS Analysis of Quarantine Insect Opogona sacchari (Lepidoptera: Hieroxestidae) from Different Hosts

Shen Jie1,Gao Qikang2,Shen Youlian3,Lou Binggan2   

  1. 1. Forest Pest Control and Quarantine Station of Zhejiang Province Hangzhou 310004; 2. Zhejiang University Hangzhou 310029;3. Cixi Forest Pest Control Station of Zhejiang Cixi 315300
  • Received:2005-05-09 Revised:1900-01-01 Online:2006-06-25 Published:2006-06-25

关键词: 蔗扁蛾, 内转录间隔区, 寄主, 检疫

Abstract:

The internal transcribed spacer (ITS) of Opogona sacchari, a important quaratine pest, from different hosts was studied by using the special primers designed by the highly conserved region sequence of 18S and 28S rDNA region, and the ITS sequences of Opogona sacchari from diffirent hosts were compared. The total length of O. sacchari ITS1, ITS2 and 5.8S rDNA is 987 bp. The average of G+C contents is 47.2%. Length of ITS1 and ITS2 fragment is 443 bp and 358 bp. The ITS1 and ITS2 from different hosts are identical or unsignificantly difference.

Key words: Opogona sacchari, internal transcribed spacer, host, quarantine