欢迎访问林业科学,今天是

林业科学 ›› 2006, Vol. 42 ›› Issue (5): 138-141.doi: 10.11707/j.1001-7488.20060525

• 研究简报 • 上一篇    下一篇

宁夏枸杞八氢番茄红素合成酶基因的克隆与序列分析

郑阳霞1,2 季静1 王罡1 杨婉身2   

  1. 1.天津大学农业与生物工程学院,天津300072;2.四川农业大学生命科学与理学院雅安625014
  • 收稿日期:2004-11-22 修回日期:1900-01-01 出版日期:2006-05-25 发布日期:2006-05-25

Cloning and Sequence Analysis of Phytoene Synthase cDNA from Lycium barbarum

Zheng Yangxia1,2,Ji Jing1,Wang Gang1,Yang Wanshen2   

  1. 1.College of Agriculture and Biotechnology,Tianjin University Tianjin 300072;2.College of Biology and Science, Sichuan Agricultural University Ya'an 625014
  • Received:2004-11-22 Revised:1900-01-01 Online:2006-05-25 Published:2006-05-25

关键词: 宁夏枸杞, 八氢番茄红素合成酶基因, 克隆

Abstract:

A novel 1.3 kb cDNA was amplified from cDNA library of Lycium barbarum by PCR.This cDNA clone was further inserted into pGEM-T. The sequence analysis showed that the cDNA was 1 292 bp long with an open reading frame of 1 275 bp, and encoded a polypeptide of 425 animo acids. Its cDNA sequence showed 75%~80% identity, and the amino acid sequence shared 70%~80% identity with those of PSY from Lycopersicon esculentum, Capsicum annuum, Arabidopsis thaliana and Zea mays respectively, indicating that the full-length PSY cDNA was isolated from L. barbarum.

Key words: Lycium barbarum, phytoene synthase, cloning