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林业科学 ›› 2006, Vol. 42 ›› Issue (1): 29-36.doi: 10.11707/j.1001-7488.20060106

• 论文及研究报告 • 上一篇    下一篇

梅花‘南京红须’花色色素花色苷的分离与结构鉴定(英文)

赵昶灵1,2 郭维明3 陈俊愉4   

  1. 1.南京农业大学生命科学学院,南京210095;2.云南农业大学农学与生物技术学院,昆明650201;3.南京农业大学园艺学院,南京210095;4.北京林业大学园林学院,北京100083
  • 收稿日期:2004-06-15 修回日期:1900-01-01 出版日期:2006-01-25 发布日期:2006-01-25

Isolation and Structural Identification of the Anthocyanins from the Flower Color Pigment of Prunus mume ‘Nanjing Hongxu’ (Nanjing Red-Bearded)

Zhao Changling1,2,Guo Weiming3,Chen Junyu4   

  1. 1.College of Life Sciences, Nanjing Agricultural University Nanjing210095; 2.College of Agricultural Sciences and Biotechnology,Yunnan Agricultural University Kunming650201; 3.College of Horticulture, Nanjing Agricultural University Nanjing210095;4.College of Landscape Architecture, Beijing Forestry University Beijing100083
  • Received:2004-06-15 Revised:1900-01-01 Online:2006-01-25 Published:2006-01-25

摘要:

梅花‘南京红须’花色色素的2种主要花色苷可用甲醇-乙酸-水(10∶1∶9)提取,再用纸层析和柱层析纯化。特征性颜色反应、薄层层析、纸层析、紫外-可见光谱、高效液相色谱、气相色谱、核磁共振谱和快原子轰击质谱分析表明:2种花色苷分别是花青素-3-氧-(6″-氧-α-吡喃型鼠李糖基-β-吡喃型葡萄糖)苷和花青素-3-氧-(6″-氧-没食子酰-3″-氧-β-吡喃型葡萄糖基-β-吡喃型葡萄糖)苷。花青苷除决定‘南京红须’的紫红花色外,还可能强化‘南京红须’在寒冷环境中的生存能力。

关键词: &lsquo, 南京红须&rsquo, 花色色素, 花色苷, 分子结构

Abstract:

Two major anthocyanins of the flower color pigment of Prunus mume‘Nanjing Hongxu’ were isolated with MeOH-HOAc-H2O (10∶1∶9, V/V), and purified by paper chromatography and subsequent column chromatography. Specific chemical reactions, TLC, PC, UV-Vis spectra, HPLC, GC, 1H NMR,13C NMR and FAB MS analyses indicate that the anthocyanins are cyanidin 3-O-(6″-O-α-rhamnopyranosyl-β-glucopyranoside) and cyanidin 3-O-(6″-O-galloyl-3″-O-β-glucopyranosyl-β-glucopyranoside) respectively. In addition to contributing to the mauve flower color, the anthocyanins may improve the ability of P. mume ‘Nanjing Hongxu’ to survive in cold.

Key words: Prunus mume “Nanjing Hongxu&rdquo, (Nanjing red-bearded);flower color pigment;anthocyanin;molecular structure