关键词: 大叶黄杨, 无芽茎段, 不定芽诱导, 不定芽起源

Abstract:

In order to improve the resistance of Euonymus japonicus, its ability of adventitious shoots regenerating from inter-node stem-segment were researched on MS medium in vitro. The effect of both plant growth regulators and carbon resources in media on adventitious shoots differentiating was studied. Regenerating system of E. japonicus was established successfully. Adventitious shoots were highly obtained from inter-node explants inoculated on MS media supplemented with 6-BA 1.7 mg·L^-1+IBA 0.005 mg·L^{-1 or 6-BA 1.9 mg·L-1 + IBA 0.03 mg·L -1. The regenerating ratio reached to 52.4% and 46.7% respectively. Media for inducing adventitious roots were 1/2MS+ IAA 0.5mg·L-1. Adventitious shoots originated from surface tissue of inter-node stem-segment.}

Key words: Euonymus japonicus, inter-node stem-segment, induction of adventitious shoots, origin of adventitious shoots