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林业科学 ›› 2004, Vol. 40 ›› Issue (5): 123-126.doi: 10.11707/j.1001-7488.20040520

• 论文及研究报告 • 上一篇    下一篇

华山松大小蠹和共生真菌分泌酶组成分析

陈辉 唐明 朱长俊 胡景江   

  1. 西北农林科技大学,杨凌712100
  • 收稿日期:2002-10-25 修回日期:1900-01-01 出版日期:2004-09-25 发布日期:2004-09-25

The Enzymes in the Secretions of Dendroctonus armandi (Scolytidae) and Their Symbiotic Fungus of Leptographium qinlingensis

Chen Hui,Tang Ming,Zhu Changjun,Hu Jingiiang   

  1. Northwest Sci Tech University of Agriculture and Forestry Yangling712100
  • Received:2002-10-25 Revised:1900-01-01 Online:2004-09-25 Published:2004-09-25

摘要:

对华山松大小蠹消化道和共生真菌胞外蛋白和酶类组成分析的结果表明,华山松大小蠹消化道和共生真菌胞外均可分泌多种酶类,其中华山松大小蠹消化道具自身分泌纤维素酶、酯酶、蛋白酶和淀粉酶的能力,但不具分泌漆酶等木质素分解酶的能力,因此,其虽然可以直接利用寄主华山松韧皮部和木质部边材组织内的纤维素、淀粉、多糖、酯类和蛋白等营养物质,但不能分解利用木质素类营养。而共生真菌除具有胞外分泌纤维素酶、酯酶、蛋白酶和淀粉酶的能力外,还能够在寄主华山松组织内木质素的诱导下分泌漆酶,以充分利用华山松韧皮部和木质部内纤维素、木质素、淀粉和脂类物质。此外,华山松大小蠹消化道和共生真菌胞外分泌的纤维素酶、酯酶、淀粉酶和蛋白酶还存在结构和营养利用方面的差异。

关键词: 华山松大小蠹, 共生真菌, 消化道, 胞外酶

Abstract:

The study of extracellular proteins and combination of enzymes of Dendroctonus armandi adult insects gut and its symbiotic fungus in the Qinling Mountains results showed that there were cellulase,hemicellulase,amylade,protease and esterase in the secretions of Dendroctonus armandi , whereas cellulase,hemicellulase,amylase,protease,esterase and laccase can be detected in symbiotic fungus At the same time,isoenzyme zymogram showed that cellulase,amylase,protease,esterase from symbiotic fungus and from the Dendroctonus armandi were difierent in the species and structure of enzyme This proved that Bark Beetle and symbiotic fungus can be utilized nourishing substances of phloem and xylem in host trees such as protein,cellulose,amylum,amylose,esters,but also symbiotic fungus can be utilized lignin in the induced lignose of host trees

Key words: Dendroctonus armandi, Symbiotic fungus, Gut, Extracellular enzyme