关键词: 木荷, 锥栗, 厚壳桂, AFLP, 遗传多样性

Abstract:

Amplified fragment length polymorphism(AFLP)was used to analyze three species: Schima superba,Castanopsis chinensis,Cryptocarya chinensis located across three different communities:coniferous community,mixed coniferous and broad-leaf community,evergreen broad-leaved community in Dinghu Mountain(112°31′39″E,23°09′21″N),China.These three communities represent three successional stages.Samples of Schima superba and Castanopsis chinensis were collected in these three communities,while samples of Cryptocarya chinensis were collected in later two communities.Individuals of each species belonging to the same community were considered as subpopulation.Four AFLP primer combinations(EcoR1,EcoR2,Mse1,Mse2)were used in total 48 individuals of Schima superba amplified 24,27,40 and 27 reliable bands,of which 15,23,23 and 16 were polymorphic respectively.The mean population genetic diversities of three subpopulations of Schima superba were 0.353,0.336 and 0.304.Similarly,total 48 individuals of Castanopsis chinensis amplified 27,20,33 and 39 reliable bands,of which 15,15,18 and 26 were polymorphic respectively.The mean population genetic diversities of three subpopulations of Castanopsis chinensis were 0.197,0.297 and 0.311.Total 40 individuals of Cryptocarya chinensis amplified 23,30,42 and 31 reliable bands,of which 12,19,37 and 18 were polymorphic respectively.The mean genetic diversities of two subpopulations of Cryptocarya chinensis were 0.285 and 0.295.These results were caused by both species biological characteristics and microenvironments of different communities.

Key words: Schima superba, Castanopsis chinensis, Cryptocarya chinensis, AFLP, Genetic diversity