替代寄主被川硬皮肿腿蜂寄生后组织结构及主要生理生化变化

doi:10.11707/j.1001-7488.20030312

林业科学 ›› 2003, Vol. 39 ›› Issue (3): 76-84.doi: 10.11707/j.1001-7488.20030312

替代寄主被川硬皮肿腿蜂寄生后组织结构及主要生理生化变化

谭毅周祖基

重庆大学生物工程学院,重庆400044；四川农业大学林学园艺学院,雅安625014

收稿日期:2001-09-11 修回日期:1900-01-01 出版日期:2003-05-25 发布日期:2003-05-25

STRUCTURAL AND MAJOR PHYSIOLOGICAL & BIOLOGICAL CHANGES OF SUBSTITUTE HOST PARASITIZED BY SCLERODERMA SICHUANENSIS XIAO(HYMENOPTERA:BETHYLIDAE)

Tan Yi,Zhou Zuji

College of Bioengineering,Chongqing University, Chongqing 400044;Department of Forestry and Horticulture, Sichuan Agricultural University Ya'an 625014

Received:2001-09-11 Revised:1900-01-01 Online:2003-05-25 Published:2003-05-25

摘要/Abstract

摘要：

替代寄主(Coleoptera)被川硬皮肿腿蜂(Scleroderma sichuanensis Xiao)雌成蜂寄生后第10d ,虫体肌肉组织的结构、与骨骼的连接、脂肪组织以及消化道、马氏管、气管等组织系统均发生了明显的变化。同时寄主机体蛋白、氨基酸含量和蛋白水解酶活力等发生了显著的变化:寄生后第3～6d ,寄主体内蛋白质含量急剧下降,第10d达最低值340.8780mg·g^-1,比寄生当天降低了17.00% ;而游离氨基酸的含量则逐渐上升,第10d达最大值22.362 7mg·g^-1,比寄生当天增加了42.45% ,其中Ser、Gly、Ala、Val、Met、Phe、Ile、Leu、Lys、Trp等10种游离氨基酸组分变化趋势与游离氨基酸总量的变化趋势一致;在所检测的17种游离氨基酸中,各寄生时期含量最高者除寄生后第1d为Tyr外,第3、6、10d均为Ala ,且其含量变化明显;而对照各期含量最高者均为Tyr。寄生后第1～3d ,蛋白酶活力迅速上升,第3d达最大值634.786 8μg Tyr·g^-1·min^-1,然后逐渐下降,到第10d接近寄生当天的水平。本文还就替代寄主的蛋白质与氨基酸代谢、川硬皮肿腿蜂的氨基酸营养生理等问题进行了讨论。

关键词: 替代寄主, 川硬皮肿腿蜂, 组织结构, 游离氨基酸, 蛋白酶

Abstract:

The structural, physiological and biochemical changes of the substitute host(Coleoptera)parasitized by Scleroderma sichuanensis were studied. The structure of the parasitized substitute host changed significantly on the 10th day. The content of protein and amino acids & the activity of proteinase changed remarkably after the substitute host parasitized by Scleroderma sichuanensis. The content of the protein decreased sharply on the 3rd to 6th day and got the minimum 340.878 0 mg·g^-1 on the 10th day after parasitization. But the total content of the free amino acids increased step by step, so got the maximum 22.362 7 mg·g^-1 on the 10th day, increased by 42.45% compared with the 1st day's. The content of Ser, Gly, Ala, Val, Met, Phe, Ile, Leu, Lys, Trp etc. changed as the total free amino acids content. The highest free amino acids content of parasitized alternative host pupae was Ala on the 3rd, 6th and 10th day, except the 1st day was Tyr with that of the control alternative host pupae being Tyr in all the period. The activity of proteinase elevated rapidly on the 1st to 3rd day after parasitization. It got the maximum 634.786 8 μg Tyr.g^-1·min^-1 on the 3rd day, then descended slowly to the level of the 1st day's. Then the metabolism of protein and amino acids of alternative host the nutritional physiology of S. sichuanensis larvae were discussed.

Key words: Substitute host, Scleroderma sichuanensis, Structure, Free amino acids, Proteinase

谭毅周祖基. 替代寄主被川硬皮肿腿蜂寄生后组织结构及主要生理生化变化[J]. 林业科学, 2003, 39(3): 76-84.

Tan Yi;Zhou Zuji. STRUCTURAL AND MAJOR PHYSIOLOGICAL & BIOLOGICAL CHANGES OF SUBSTITUTE HOST PARASITIZED BY SCLERODERMA SICHUANENSIS XIAO(HYMENOPTERA:BETHYLIDAE)[J]. Scientia Silvae Sinicae, 2003, 39(3): 76-84.

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替代寄主被川硬皮肿腿蜂寄生后组织结构及主要生理生化变化

STRUCTURAL AND MAJOR PHYSIOLOGICAL & BIOLOGICAL CHANGES OF SUBSTITUTE HOST PARASITIZED BY SCLERODERMA SICHUANENSIS XIAO(HYMENOPTERA:BETHYLIDAE)

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