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林业科学 ›› 2003, Vol. 39 ›› Issue (2): 102-108.doi: 10.11707/j.1001-7488.20030217

• 论文及研究报告 • 上一篇    下一篇

刚竹毒蛾白僵菌优良菌株筛选及生产应用研究

蔡国贵   

  1. 福建省森林病虫害防治检疫总站,福州350003
  • 收稿日期:2001-08-22 修回日期:1900-01-01 出版日期:2003-03-25 发布日期:2003-03-25

SCREENING OF THE SUPERIOR STRAINS OF BEAUVERIA BASSIANA OF PANTANA PHYLLOSTACHYSAE AND PRACTICAL APPLICATION

Cai Guogui   

  1. General Station of Forest Diseases and Insect Pests Control and Quarantine of Fujian Province Fuzhou?350003
  • Received:2001-08-22 Revised:1900-01-01 Online:2003-03-25 Published:2003-03-25

摘要:

本研究对天然刚竹毒蛾僵虫蛹分离纯化的8个球孢白僵菌菌株,进行了菌落形态、菌落生长、产孢量和发芽率等培养性状测定,并与4个马尾松毛虫球孢白僵菌菌株进行了室内外防治刚竹毒蛾试验比较,结果表明:菌株间的培养性状和毒力有明显差异,各菌株存在较明显的寄主专化性,分离自刚竹毒蛾的菌株毒力较高。筛选出BP4和BP1 2个优良菌株,具有菌丝生长速度快,产孢时间短,产孢能力强,毒力高等优点,并将最优的BP4菌株应用于生产,菌粉平均含孢量达238.7×108·g-1,平均孢子发芽率达96.2% ,林间防治越冬代刚竹毒蛾平均效果达92.3% ,取得良好的防治效果。

关键词: 刚竹毒蛾, 球孢白僵菌, 菌株筛选, 应用

Abstract:

strains, isolated from the cadaver of Pantana phyllostachysae infected by Beauveria bassiana,were studied on their colony morphology, growth, sporulation and germination; and their capabilities for controlling Pantana phyllostachysae indoors and outdoors were compared with 4 strains of Beauveria bassiana of Dendrolimus punctatus. The results indicated that the strains had significant difference in their cultural characters, virulence and specialization. The 8 strains were highly virulent to Pantana phyllostachysae and two superior strains, BP4 and BP1, were obtained. The mycelium of the two strains grew fast and could produce large amount of spores with high virulence. The superior strain BP4 was applied in mass production, with 238.7×108·g-1 sporulation on the average, 96.2% germination and 92.3% efficiency for controlling Pantana phyllostachysae in bamboo forest.

Key words: Pantana phyllostachysae, Beauveria bassiana, Strain screening, Application