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林业科学 ›› 2002, Vol. 38 ›› Issue (6): 49-52.doi: 10.11707/j.1001-7488.20020609

• 论文及研究报告 • 上一篇    下一篇

乐昌含笑不同类型鉴定的ISSR-PCR分析

邱英雄 傅承新 何云芳   

  1. 浙江大学生命科学学院,杭州310029;浙江省林业厅种苗管理站,杭州310029
  • 收稿日期:2001-08-27 修回日期:1900-01-01 出版日期:2002-11-25 发布日期:2002-11-25

IDENTIFICATION OF MICHELIA TSOI TYPES USING ISSR-PCR MARKER ASSAYS

Qiu Yingxiong,Fu Chengxin,He Yunfang   

  1. College of Life Sciences, Zhejiang University Hangzhou310029;Management Station of Seed and Seedling,Zhejiang Forestry Bureau Hangzhou310029
  • Received:2001-08-27 Revised:1900-01-01 Online:2002-11-25 Published:2002-11-25

摘要:

利用inter -简单重复序列(ISSR)标记对乐昌含笑6种类型的遗传变异进行了研究,从30个引物中筛选出12个多态性引物用于正式扩增,共扩增出134条DNA带,其中多态性DNA带67条,占50% ,平均每个引物扩增的DNA带的数目为11.167条。其中引物ISSR16、ISSR19能区分全部类型。引物ISSR16、ISSR19和ISSR2在不同的类型中扩增出了一些特有条带,对乐昌含笑类型和品种鉴定以及检验品种的真实性方面非常有价值。本研究对ISSR分析技术的关键步骤进行了讨论,并利用DNA扩增结果对供试类型进行了聚类分析。

关键词: 乐昌含笑, 类型鉴定, ISSR-PCR

Abstract:

Based on introduction and selective breeding of Michelia tsoi, genetic variations of 6 M. tsoi types were determined using ISSR marker assays. 12 primers were screened from 30 primers, and total 134 DNA bands were amplified, 67 of which (50%) were polymorphic. The average number of DNA bands amplified by each primer was 11.167. DNA profiles based on ISSR16, ISSR19 and ISSR2 have revealed potential diagnostic fingerprints and specific bands for various types. The ISSR specific bands are useful for the identification of cultivated germplasm, and testing trueness of cultivars. Key steps of ISSR-PCR analysis techniques and application to testing trueness and purity of M. tsoi types were discussed. A dendrogram was constructed by using UPGMA algorithm.

Key words: Michelia tsoi Dandy, Types identification, ISSR-PCR