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林业科学 ›› 2002, Vol. 38 ›› Issue (4): 46-52.doi: 10.11707/j.1001-7488.20020408

• 论文及研究报告 • 上一篇    下一篇

不同生长素处理对桉树无性系插条氧化酶活性影响的比较研究

黄卓烈 李明 詹福建 谭绍满   

  1. 华南农业大学生命科学学院,广州510642;华南农业大学林学院,广州51064
  • 收稿日期:2000-08-07 修回日期:1900-01-01 出版日期:2002-07-25 发布日期:2002-07-25

STUDY ON THE COMPARISON OF THE CHANGES OF OXIDASE ACTIVITIES IN EUCALYPTUS CUTTINGS TREATED WITH DIFFERENT AUXINS

Huang Zhuolie,Li Ming,Zhan Fujian,Tan Shaoman   

  1. College of Life Science, South China Agricultural University Guangzhou 510642;College of Forestry, South China Agricultural University Guangzhou510642
  • Received:2000-08-07 Revised:1900-01-01 Online:2002-07-25 Published:2002-07-25

摘要:

尾叶桉MLA无性系(简称MLA)是扦插难生根无性系,尾叶桉U6 无性系(简称U6)和刚果12号桉W5无性系(简称W5)是相对易生根无性系。分别用吲哚乙酸(IAA)、吲哚丁酸(IBA)、萘乙酸(NAA)处理MLA、U6 和W5插条后,各无性系插条体内的过氧化物酶(POD)、多酚氧化酶(PPO)、吲哚乙酸氧化酶(IAAO)活性呈现规律性的升高。在生根过程的不同时期,各酶的活性差异极显著,表明桉树插条的不定根发生和发展与POD、PPO、IAAO活性有密切联系。而IAA、IBA、NAA对不同无性系和不同的氧化酶活性的促进效果也有显著差异。讨论了生长素促进桉树插条氧化酶活性和不定根发生与发展的机理。

关键词: 桉树, 扦插生根, 生长素, 氧化酶

Abstract:

Eucalyptus urophylla MLA clone(MLA) was difficult-to-root clone. E.urophylla U6 clone(U6) and E. ABL No.12 W5 clone(W5) were relatively easy-to-root clones. After the cuttings of MLA, U6 and W5 were treated with indoleacetic acid(IAA), indolebutyric acid(IBA), and nathphylacetic acid(NAA), the activities of peroxidase (POD), polyphenol oxidase(PPO) and indoleacetic acid oxidase(IAAO) increased regularly. The differences of enzyme activities in different stages of rooting were very significant. It was indicated that the origin and development of adventitious roots in Eucalyptus were relative to POD,PPO, and IAAO. The differences of the promoting efficiency of IAA, IBA, and NAA to different clones and different oxidase activities were significant or very significant. The mechanism of promoting oxidase activities and promoting rooting of Eucalyptus cuttings by auxins was discussed.

Key words: Eucalyptus, Rooting of cuttings, Auxins, Oxidases