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林业科学 ›› 2002, Vol. 38 ›› Issue (2): 68-72.doi: 10.11707/j.1001-7488.20020212

• 论文及研究报告 • 上一篇    下一篇

牛心朴和苦楝果提取物对桑天牛产卵及卵孵化的抑制作用

赵博光1 陈小平3 巨云为4 李小平2   

  1. 南京林业大学,南京210037;安徽淮北煤炭师范学院,淮北235000
  • 收稿日期:2001-03-01 修回日期:1900-01-01 出版日期:2002-03-25 发布日期:2002-03-25

INHIBITING EFFECTS OF THE CYNANCHUM KOMALOVII TOTAL ALKALOIDS AND MELIA AZEDARACH FRUIT EXTRACTS ON OVIPOSITION AND EGG HATCH IN APRIONA GERMARI (COLEOPTERA, CERAMBACIDAE)

Zhao Boguang1,Cheng Xiaoping3,Ju Yunwei4,Li Xiaoping2   

  1. Nanjing Forestry University Nanjing 210037;Anhui Huaibei Normal College Huaibei 235000
  • Received:2001-03-01 Revised:1900-01-01 Online:2002-03-25 Published:2002-03-25

摘要:

使用一定浓度的牛心朴总生物碱苦楝果提取物处理的构树枝条作为天牛成虫的食物,比较了其对桑天牛产卵量及卵孵化率的影响。在室温下,将成对的天牛放在盖有铁纱网的直径25cm高25cm的塑料桶中进行试验。在15d试验中,用300μg·mL-1 的牛心朴总生物碱、1000μg·mL-1 的苦楝果提取物处理枝条的两处理组的各7对天牛其日平均产卵量分别为3.53±4.18个和6.73±3.82个,而对照组7对天牛的日均产卵量为13.73±2.90个。经t—检验处理组与对照组之间在日均产卵量方面差异极为显著。300μg·mL-1 的牛心朴总生物碱和1000μg·mL-1 的苦楝果提取物对桑天牛卵孵化有显著影响。其影响表现在两方面:(1)孵化率降低。成虫取食300μg·mL-1 牛心朴总生物碱和1000μg·mL-1 苦楝果提取物处理的构树枝条后产的卵最终孵化率分别为77.36%、87.12%。而对照组的最终卵孵化率为99.5%。(2)其孵化期延长。牛心朴处理组的最终孵化期为17天、苦楝果处理组的最终孵化期为15d、而对照组的最终孵化期为11d。本文还结合我国牛心朴和苦楝的资源情况讨论了利用它们的提取物研究开发防治害虫的行为控制新方法。

关键词: 牛心朴, 苦楝, 提取物, 桑天牛, 产卵, 孵化

Abstract:

The branches of Broussonetia papyrifera were treated with Cynancum komalovii total alkaloids and Melia azedarach fruit extracts as the food of the beetle (Apriona germari Hope), the effects of C.komalovii total alkaloids and M.azedarach fruit extracts on their oviposition and egg hatch were investigated and compared. The testing paired beetles were put into the plastic containers with diameter of 25 cm and 25 cm in height and covered with iron screen. The experiments were carried in laboratory at the room temperature. During 15 d testing period the average egg numbers laid per d of the treated seven pairs of the beetles in the two treated groups, which were fed on branches treated by 300 μg·mL-1 C.komalovii total alkaloids and 100 μg·mL-1 M.azedarach fruit extracts were 3.53±4.18 and 6.73±3.82 respectively and in the control group it was 13.73±2.90. t-test showed that there were significant differences between the treated and control groups in average egg number per d. The Cynancum komalovii total alkaloids and the Melia azedarach fruit extracts affected egg hatching in A.germari, which were shown in two aspects:(1) The egg hatch rate was lowered. the final egg hatch rate in the group of 300 μg·mL-1 C.komalovii total alkaloids and in the group of 1000 μg·mL -1 M.azedarach fruit extracts were 77.36%,87.12% respectively, and that in control group was 99.5%. (2) The time period needed for egg hatch was increased. In the group of 300 μg·mL-1 C.komalovii total alkaloids, the time for egg hatch was 17d, in the group of 1000 μg·mL-1 M.azedarach fruit extracts it was 15 d and that was 11 d in the control group.In brief, in the experiment it was shown that oviposition and egg hatch were significantly inhibited for the adults of Apriona germari, which had been fed with C.komalovii total alkaloids and M. azedarach fruit extracts. To develop new ways of behavior Manipulation for Insect Pest Management with the extracts of C.komalovii and M.azedarach were discussed with resources of the two plant species in China in the paper.

Key words: Cynancum komalovii<, .i>, Al. Iljinski, Melia azedarach Linn., extracts, Apriona germari Hope, oviposition, hatch