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林业科学 ›› 2000, Vol. 36 ›› Issue (5): 54-58.doi: 10.11707/j.1001-7488.20000517

• 论文及研究报告 • 上一篇    下一篇

落叶松—杨栅锈菌遗传分化的RAPD分析

田呈明 康振生 李振岐 赵彦修 张慧   

  1. 西北农林科技大学,杨陵712100;山东师范大学,济南250014
  • 收稿日期:1999-09-13 修回日期:1900-01-01 出版日期:2000-09-25 发布日期:2015-04-23

ANALYSIS OF THE GENETIC SPECIALIZATION OF MELAMPSORA LARICI-POPULINA WITH RAPD MARKERS

Tian Chengming,Kang Zhensheng,Li Zhenqi,Zhao Yanxiu,Zhang Hui   

  1. Northwestern Sci-Tech University of Agriculture and Forestry Yangling 712100;Shandong Normal University Jinan250014
  • Received:1999-09-13 Revised:1900-01-01 Online:2000-09-25 Published:2015-04-23

摘要: 用随机扩增多态性DNA(Random amplified polymorphic DNA ,RAPD)技术对来自陕西及青海的7个地区的1 3个落叶松—杨栅锈菌(Melampsora larici-populina Kleb.)的分离物进行了基因组DNA多态性分析。13个10 - 核苷酸随机引物(Operon公司)对13个菌株共扩增出81条RAPD带,其中69个DNA片断呈现多态性,占总扩增片断的85.2 %。供试菌株的相似系数在0.608~1.000之间,各菌株之间的差异在0~33.1%之间,并建立了聚类树状图。13个菌株在相似性76.1%时被分为4个类群:I组包括秦岭宁陕火地塘C的2个分离物,II组为火地塘B的1个分离物;III组为青海西宁、互助,陕西太白宝太路、宝鸡天台山、周至厚畛子(HZa)等5个地区的8个分离物;第IV组为陕西周至厚畛子的2个菌株(HZb、HZc)。并与各菌株对不同杨树致病性的聚类分析进行了比较,结果表明各菌株间的DNA多态性与致病性及寄主、地理来源等没有明显相关性。

关键词: 杨树, 落叶松-杨栅锈菌, RAPD, 致病性

Abstract: The genomic DNA polymorph on 13 isolator of Melampsora larici-populina Kleb. from Shaanxi and Qinghai were analyzed using random amplified polymorphism DNA markers (RAPD). 81 RAPD bands were amplified with 13 decanucleotide random primers, 69 bands of them were polymorphic(85.2%).Similarity coefficient of isolates was 0.608~1.000 and difference among isolates was 0~33 1%. The tested 13 isolates of the group were divided into 4 clusters with the similarities and genetic distances among them. Two isolates from Huoditang C in Qingling were placed in one group(group I) and one isolate from Huoditang B in another group(group II). Six isolates from Xining, Huzhu in Qinghai and Taibeibaotailu, Tiantaishan, Houzhengzi(Hza) in Shaanxi were placed in the third group(group III). Last two isolates from Houzhengzi(Hza,HZb) were placed in the fourth group(group IV).However, the DNA polymorphic of isolations were not obviously related to the collecting places or hosts and pathogenicity.

Key words: Poplar, %Melampsora larici-populina, RAPD, Pathogenicity