The latex of Euphorbia tirucalli from Xishuangbanna and Hainan Island were analysed by agilent gas chromatography/mass spectrometry.52 peaks (42 peaks,E.tirucalli from Hainan) were seperated and 33 peaks were identified, the latex contains as hydrocarbons normal and isomeric alkanes with carbon numbers from 9~36, eg, 2,6-dimethyl-nonane,3,7-dimethyl-decane, hexatriacontane, pentadecane, dodecane, heptadecane, eicosane, tricosane,heneicosane, triacontane, docosane, N-eicosane,caryophyllene, 1,2-benzenedicarboxylic acid,ester and sulfur etc, except main constitutents “fingerprint" Lanosta-8,24-dien-3-ol.The alkanes represent 14.57% of the total content.The components of latex not exist in significant differences between two E. tirucalli from Xishuangbannan and Hainan Island.The main components of younger tree from Hainan also contain as hydrocarbons normal and isomeric alkanes with carbon numbers from 9~36,eg,eicosane,2,4-dimethyl-,octadecane,cyclohexasiloxane,dodecamethyl,nonadecane,pentaco-sane,heneicosane,eicosane, hexadecane, heptadecane, docosane, N-eicosane,beta-cubebene, caryophyllene, germacrene-D, 1,2-benzenedicarboxylic acid,ester and sulfur compound etc, but the content of Lanosta-8,24-dien-3β-ol(17.40%) reprsents higher than the content of adult tree(10.91%)from Xishuangbanna, lower than adult tree on alkanes, the alkanes of younger tree represent 4.86%, adult tree represent 14.57% of the total content.