菌根真菌调控灌木铁线莲根际土壤生态化学计量特征对氮沉降的应激响应

doi:10.11707/j.1001-7488.20220615

Abstract

Abstract:

Objective: The responses of rhizosphere soil stoichiometry of 1-year-old mycorrhizal and non-mycorrhizal Clematis fruticosa seedlings in relation to nitrogen (N) deposition were investigated. The regulatory mechanisms of mycorrhizal biotechnology on micro-ecological environment in rhizosphere soil were analyzed to provide a theoretical basis for exploring the stability of ecosystem under the background of global climate change. Method: The 1-year-old mycorrhizal and non-mycorrhizal C. fruticosa seedlings were selected for pot experiments. The inoculation treatments included single inoculation (inoculated with Rhizophagus intraradices or Funneliformis mosseae), mixed inoculation (inoculated with the mixed fungi inoculum of R. intraradices and F. mosseae with the mass ratio of 1∶1), and no inoculation as the control. Four N deposition treatments were established: 0N (0N, 0 g·m^-2a^-1); low N (LN, 3 g·m^-2a^-1); medium N (MN, 6 g·m^-2a^-1); and high N (HN, 9 g·m^-2a^-1). The soil available nutrients stoichiometry, microbial biomass stoichiometry, ecoenzymatic stoichiometry, and microbial nutrient limitation (i.e., vector length represents the microbial C relative limitation, vector angle represents the microbial N or P relative limitation) were measured, in order to explore the regulatory mechanisms of microbial metabolism in rhizosphere soil in response to AMF inoculation and N deposition treatments. Result: 1) Comparing with the control, all of the AMF inoculation treatments increased the ratio of dissolved organic C and available N, and the ratio of dissolved organic C and available P in the rhizosphere soil under all N deposition treatments. The ratio of available N and available P in HN treatment was the highest and significantly higher than that in LN treatment in all of the AMF inoculation treatments. The ratio of available N and available P in MN treatment was the highest and significantly higher than that in LN treatment in the control. 2) Under 0N treatment, the ratio of microbial biomass N and microbial biomass P in the control was significantly higher than that in all of the AMF inoculation treatments. Under MN and HN treatments, the ratio of microbial biomass N and microbial biomass P in the control was notably lower than that in all of the AMF inoculation treatments. Under LN treatment, the ratio of microbial biomass C and microbial biomass N in the F. mosseae inoculation treatment was significantly increased by 208.5%, 109.2% and 209.4% than that in the non-inoculated, R. intraradices inoculation and mixed inoculation treatments, respectively. 3) Under 0N treatment, the stoichiometry of enzymes related to C and N acquisition, the stoichiometry of enzymes related to C and P acquisition, vector angle and vector length in all of the AMF inoculation treatments were significantly higher than those in the control. Under HN treatment, there was no significant differences of vector length among all of the four inoculation treatments. 4) The total effects of microbial C and P limitations of inoculation treatments was higher than that of N deposition treatments. The inoculation treatments significantly increased the microbial C and P limitations. Conclusion: AMF inoculation effectively regulates the responses of stoichiometry in rhizosphere soil to low N deposition, and the ability of the regulation of F. mosseae was the best. The regulatory effect of AMF inoculation was greater than nitrogen deposition on the microbial C and P limitations in rhizosphere soil.

Key words: arbuscular mycorrhizal fungi, nitrogen deposition, available nutrients stoichiometry, microbial biomass stoichiometry, ecoenzymatic stoichiometry, microbial nutrient limitation

CLC Number:

S718.8

Longfei Hao,Tingyan Liu,Yongqin He,Shengxi Zhang,Yuan Zhao. Responses of Rhizosphere Soil Stoichiometry of Clematis fruticosa Inoculated with Arbuscular Mycorrhizal Fungi to Nitrogen Deposition[J]. Scientia Silvae Sinicae, 2022, 58(6): 151-160.

Figures/Tables 7

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施氮序号 Sequence number of nitrogen addition	不施氮 No nitrogen treatment(0N)	低氮处理 Low nitrogen treatment(LN)	中氮处理 Medium nitrogen treatment(MN)	高氮处理 High nitrogen treatment(HN)
1	0	2.2	4.2	6.3
2	0	2.2	4.2	6.3
3	0	3.5	7.2	10.7
4	0	3.5	7.2	10.7
5	0	7.5	15.2	22.7
6	0	7.5	15.2	22.7
7	0	8.3	16.7	25.0
8	0	8.3	16.7	25.0
9	0	3.5	6.8	10.3
10	0	3.5	6.8	10.3

处理Treatments		DOC∶AN	DOC∶AP	AN∶AP
-M	0N	1.01±0.03 fg	3.14±0.11 g	3.12±0.11 h
	LN	0.80±0.06 gh	2.92±0.19 g	3.64±0.09 fg
	MN	0.50±0.03 i	3.01±0.10 g	6.07±0.22 a
	HN	0.48±0.01 i	2.26±0.07 h	4.72±0.03 cd
+R	0N	1.36±0.10 bcd	5.69±0.20 bc	4.21±0.16 de
	LN	1.12±0.04 ef	4.19±0.23 f	3.72±0.12 ef
	MN	1.34±0.08 bcd	4.82±0.24 de	3.58±0.04 fg
	HN	1.01±0.05 fg	4.57±0.19 ef	4.52±0.04 d
+F	0N	1.66±0.10 a	4.68±0.08 ef	2.83±0.13 h
	LN	1.50±0.12 ab	4.91±0.11 de	3.31±0.24 fgh
	MN	1.65±0.14 a	4.90±0.20 de	3.00±0.13 h
	HN	1.17±0.00 def	6.06±0.30 ab	5.20±0.28 bc
+RF	0N	1.40±0.02 bc	4.68±0.08 ef	3.34±0.08 fgh
	LN	1.26±0.03 cde	6.41±0.12 a	5.11±0.23 bc
	MN	0.96±0.01 fgh	5.34±0.26 cd	5.53±0.23 b
	HN	0.79±0.03 h	4.92±0.06 de	6.24±0.26 a
接菌处理Inoculation treatment		^***	^***	^***
氮沉降处理Nitrogen deposition		^***	NS	^***
接菌处理×氮沉降处理 Inoculation×nitrogen desposition treatment		^***	^***	^***

处理Treatments		C∶N_Mic	C∶P_Mic	N∶P_Mic
-M	0N	2.82±0.55 d	9.04±1.72 h	3.22±0.04 b
	LN	10.57±0.22 cd	15.71±0.20 fg	1.49±0.05 d
	MN	17.48±0.32 c	18.17±1.65 ef	1.04±0.09 de
	HN	8.56±0.24 cd	11.92±1.69 dh	1.39±0.15 d
+R	0N	18.58±1.52 c	15.38±1.20 fg	0.83±0.01 ef
	LN	15.59±0.67 c	21.52±0.71 de	1.38±0.01 d
	MN	17.35±1.65 c	41.72±1.99 a	2.43±0.13 c
	HN	11.58±0.43 cd	27.08±1.60 bc	2.34±0.16 c
+F	0N	64.70±11.58 a	13.72±0.73 fgh	0.22±0.03 g
	LN	32.61±0.51 b	12.08±1.66 gh	0.37±0.06 fg
	MN	9.11±0.36 cd	22.56±0.23 bcde	2.48±0.08 c
	HN	5.16±0.04 d	12.73±1.55 gh	2.47±0.32 c
+RF	0N	8.74±0.41 cd	18.64±1.48 ef	2.14±0.17 c
	LN	10.54±0.69 cd	22.35±2.33 cde	2.12±0.18 c
	MN	9.40±0.86 cd	24.47±2.55 bcd	2.60±0.13 c
	HN	2.96±0.22 d	27.78±2.81 b	9.36±0.42 a
接菌处理Inoculation treatment		^***	^***	^***
氮沉降处理Nitrogen deposition		^***	^***	^***
接菌处理×氮沉降处理 Inoculation×nitrogen desposition treatment		^***	^***	^***

处理 Treatments		C∶N_EEA	C∶P_EEA	N∶P_EEA	Vector L	Vector A
-M	0N	0.13±0.03 f	0.15±0.03 d	1.16±0.01 a	0.19±0.04 f	40.70±0.33 j
	LN	0.19±0.03 f	0.21±0.03 cd	1.13±0.04 a	0.29±0.04e f	41.47±0.99 ij
	MN	0.38±0.06 ef	0.40±0.06 ab	1.06±0.03 b	0.56±0.08 de	43.39±0.75 hi
	HN	0.51±0.09 de	0.44±0.06 ab	0.86±0.03 d	0.67±0.11 cd	49.27±0.97 f
+R	0N	0.52±0.06 de	0.50±0.03 ab	0.96±0.05 c	0.72±0.06 cd	46.15±1.36 g
	LN	0.37±0.08 ef	0.38±0.07 ab	1.04±0.02 b	0.54±0.11 de	43.87±0.61 h
	MN	0.47±0.07 de	0.40±0.04 ab	0.84±0.03 d	0.62±0.08 cd	49.81±1.02 f
	HN	0.61±0.06 cde	0.33±0.04 bc	0.53±0.02 fg	0.70±0.07 cd	62.25±0.88 bc
+F	0N	0.68±0.07 bcd	0.36±0.04 bc	0.53±0.01 fg	0.77±0.08 bcd	62.00±0.44 bc
	LN	0.69±0.03 bcd	0.48±0.01 ab	0.69±0.02 e	0.84±0.04 bcd	55.41±0.74 e
	MN	0.60±0.16 cde	0.37±0.09 ab	0.63±0.01 e	0.71±0.18 cd	57.89±0.52 d
	HN	0.50±0.06 de	0.33±0.04 bc	0.67±0.00 e	0.60±0.07 d	56.11±0.04 de
+RF	0N	1.09±0.08 a	0.53±0.04 a	0.49±0.00 fg	1.21±0.09 a	63.95±0.25 ab
	LN	1.08±0.14 a	0.50±0.06 ab	0.47±0.01 g	1.19±0.16 a	64.89±0.46 a
	MN	0.92±0.11 ab	0.50±0.04 ab	0.55±0.02 f	1.05±0.11 ab	61.13±0.83 c
	HN	0.79±0.09 bc	0.49±0.05 ab	0.63±0.00 e	0.93±0.10 abc	57.88±0.11 d
接菌处理 Inoculation treatment		^***	^***	^***	^***	^***
氮沉降处理 Nitrogen deposition		NS	NS	^***	NS	^***
接菌处理×氮沉降处理 Inoculation×nitrogen desposition treatment		^**	^**	^***	^*	^***

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Responses of Rhizosphere Soil Stoichiometry of Clematis fruticosa Inoculated with Arbuscular Mycorrhizal Fungi to Nitrogen Deposition

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