Scientia Silvae Sinicae ›› 2022, Vol. 58 ›› Issue (3): 69-78.doi: 10.11707/j.1001-7488.20220308
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Lüji Wang,Xiaoxiao Zhang,Jun Wang*
Received:
2021-04-16
Online:
2022-03-25
Published:
2022-06-02
Contact:
Jun Wang
CLC Number:
Lüji Wang,Xiaoxiao Zhang,Jun Wang. Chromosome Behaviors Tracking during Meiosis of Microsporocytes of Allotriploid Populus alba × P. berolinensis 'Yinzhong' Based on 45S rDNA-FISH[J]. Scientia Silvae Sinicae, 2022, 58(3): 69-78.
Table 1
Enzymatic hydrolysis effects of different cellulase and pectinase concentrations on the anthers of Populus alba × P. berolinensis 'Yinzhong'"
处理 Treatment | 酶液浓度 Enzyme concentration (%) | 细胞分散效果 Effect of cell spread | |
纤维素酶 Cellulase | 果胶酶 Pectolyase | ||
1 | 0 | 0 | - |
2 | 0 | 1 | - |
3 | 0 | 2 | - |
4 | 0 | 3 | - |
5 | 1 | 0 | - |
6 | 1 | 1 | - |
7 | 1 | 2 | + |
8 | 1 | 3 | + |
9 | 2 | 0 | + |
10 | 2 | 1 | + + |
11 | 2 | 2 | + + |
12 | 2 | 3 | + + |
13 | 3 | 0 | + |
14 | 3 | 1 | + + + |
15 | 3 | 2 | - |
16 | 3 | 3 | - |
Fig.1
Meiotic chromosome preparation of Populus alba × P. berolinensis 'Yinzhong' pollen mother cells (PMCs) treated with different enzymes a-g: Pollen mother cell were digested with 1% cellulase +2% pectinase at 37 ℃ for 2.5 h; h: Pollen mother cell ruptured by excessive enzymolysis; i-p: Pollen mother cells were digested with 3% cellulase+1% pectinase at 37 ℃ for 3 h. Bars are equal to 10 μm."
Table 2
Distribution of 45S rDNA signals in different stages of meiosis in pollen mother cells (PMCs) of Populus alba × P. berolinensis 'Yinzhong'"
减数分裂时期 Meiotic stage | 分析细胞数量 Number of analyzed PMCs | 信号分布类型 Signal distribution type | 细胞数量 Number of PMCs | 百分率 Percentage (%) | 减数分裂时期 Meiotic stage | 分析细胞数量 Number of analyzed PMCs | 信号分布类型 Signal distribution type | 细胞数量 Number of PMCs | 百分率 Percentage (%) | |
中期Ⅰ Metaphase Ⅰ | 153 | Ⅲ | 106 | 69.28 | 2/1/0/0 | 1 | 1.92 | |||
Ⅱ+Ⅰ | 43 | 28.10 | 1/1/1/1 | 2 | 3.85 | |||||
Ⅰ+Ⅰ+Ⅰ | 4 | 2.61 | 1/1/1/0 | 1 | 1.92 | |||||
后期Ⅰ Anaphase Ⅰ | 118 | 2/1 | 99 | 83.90 | 1/1/0/0 | 1 | 1.92 | |||
3/0 | 4 | 3.39 | 末期Ⅱ Telophase Ⅱ | 100 | 2/2/1/1 | 63 | 63.00 | |||
1/1+1 | 7 | 5.93 | 2/2/2/0 | 1 | 1.00 | |||||
2/0+1 | 4 | 3.39 | 3/2/1/0 | 2 | 2.00 | |||||
1/0+2 | 4 | 3.39 | 2/1/1/1+1 | 4 | 4.00 | |||||
末期Ⅰ Telophase Ⅰ | 61 | 2/1 | 57 | 93.44 | 2/2/1/0+1 | 1 | 1.00 | |||
1/1 | 2 | 3.28 | 2/1/1/1 | 7 | 7.00 | |||||
2/0 | 1 | 1.64 | 2/2/1/0 | 1 | 1.00 | |||||
1/0 | 1 | 1.64 | 2/2/0/0 | 1 | 1.00 | |||||
中期Ⅱ Metaphase Ⅱ | 107 | 2/1 | 86 | 80.37 | 2/1/1/0 | 2 | 2.00 | |||
3/0 | 2 | 1.87 | 1/1/1/1 | 6 | 6.00 | |||||
2/0+1 | 3 | 2.80 | 1/1/0/0 | 1 | 1.00 | |||||
1/1+1 | 4 | 3.74 | 3/2/1 | 1 | 1.00 | |||||
1/1 | 4 | 3.74 | 2/2/2 | 1 | 1.00 | |||||
2/0 | 3 | 2.80 | 4/1/1 | 1 | 1.00 | |||||
1/0 | 3 | 2.80 | 3/1/1 | 1 | 1.00 | |||||
0/0+2 | 1 | 0.93 | 4/2 | 2 | 2.00 | |||||
1/0+1 | 1 | 0.93 | 3/3 | 3 | 3.00 | |||||
后期Ⅱ Anaphase Ⅱ | 52 | 2/2/1/1 | 38 | 73.08 | 4/1 | 1 | 1.00 | |||
3/1/1/1 | 3 | 5.77 | 3/2+1 | 1 | 1.00 | |||||
2/2/1/0+1 | 3 | 5.77 | ||||||||
2/1/1/1+1 | 3 | 5.77 |
Fig.2
Distribution of 45S rDNA signals in different stages of meiosis I in pollen mother cells (PMCs) of Populus alba × P. berolinensis 'Yinzhong' a: Pachytene; b-d: Metaphase Ⅰ; b: Signals localized on a trivalent; c: Signals localized on 1 univalent and 1 bivalent; d: Signals localized on 3 univalents; e-g: Anaphase Ⅰ; e: 2 signals localized on one pole and 1 on the other; f: 2 signals on one pole and the other on a lagging chromosome; g: 3 signals are localized on the same pole and the other is no signal; h-i: Telophase Ⅰ; h: 2 signals on 1 daughter nucleus and 1 signal on the other; i: 1 signal in each of the two daughter nucle i. 45S rDNA signal in green. The white arrow indicates the lagging chromosome where the signal is located. Bars are equal to 10 μm."
Fig.3
Distribution of 45S rDNA signals in different stages of meiosis Ⅱ in pollen mother cells (PMCs) of Populus alba × P. berolinensis 'Yinzhong' a-c: Metaphase Ⅱ; a: 2 signals on one set of chromosomes and 1 on another; b: Each of the 2 sets of chromosomes has 1 signal, and the other signal is located on the micronucleus chromosome in the cytoplasm; c: 2 signals on 1 set of chromosomes, 1 signal on another set of chromosomes, metaphase Ⅱ cells with tripolar spindle; d-f: Anaphase Ⅱ; d: 4 spindles have 2 signals, 2 signals, 1 signal and 1 signal respectively; e: 2 signals are located at one pole of the spindle, the other three spindle poles each have 1 signal, and 1 signal is located on the backward chromosome; f: Each pole has 1 signal; g-l: Telophase Ⅱ; g: There are 2, 2, 1 and 1 signals in each daughter nucleus; h: There are 2, 1, 1 and 1 signals in each daughter nucleus; i: There is 1 signal in each daughter nucleus; j: There are 2, 1, 1 and 1 signals in each subnuclei, and the other signal is located on the micronucleus; k: 3 signals in each of the two daughter nuclei; l: 3, 2 and 1 signals in each of the three daughter nuclei. Green is the 45S rDNA signal. The white arrow indicates the lagging chromosome or micronucleus where the signal is located. Bars are equal to 10 μm."
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