Scientia Silvae Sinicae ›› 2021, Vol. 57 ›› Issue (1): 30-39.doi: 10.11707/j.1001-7488.20210104
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Xiaoxue Wu,Aijing Zhang,Ying Gai,Xiangning Jiang*
Received:
2020-02-21
Online:
2021-01-01
Published:
2021-03-10
Contact:
Xiangning Jiang
CLC Number:
Xiaoxue Wu,Aijing Zhang,Ying Gai,Xiangning Jiang. Effects of Exogenous Hormones on Different Stages of Somatic Embryogenesis of Larix kaempferi[J]. Scientia Silvae Sinicae, 2021, 57(1): 30-39.
Fig.2
Morphological observation of somatic embryogenesis of Larix kaempferi A: Proembryogenic mass for 14 days; B: FDA staining and morphological observation of proembryogenic mass for 14 days; C: Early globular embryo; D: Middle globular embryo; E: Late globular embryo; F: Suspension cells for 14 days; G: FDA staining and morphological observation of suspension cells for 14 days; H: Early cotyledon-shaped embryo; I: Middle cotyledon-shaped embryo; J: Late cotyledon-shaped embryo."
Table 3
Effects of exogenous hormones on proliferation and somatic embryogenesis of proembryogenic masses for Larix kaempferi"
激素 Hormone | 浓度 Concentration/(mg·L-1) | 胚性胚柄团鲜质量 Fresh weight of proembryogenic masses/g | 体胚发生数量 Number of somatic embryos/(embryo·g-1) | |||||||||
7 d | 14 d | 21 d | 均值 Mean | 极差 Range | ||||||||
均值 Mean | 极差 Range | 均值 Mean | 极差 Range | 均值 Mean | 极差 Range | |||||||
2, 4-D | 0.15 | 0.89 a | 0.35 | 2.28 a | 1.62 | 3.12 a | 2.32 | 87.62 a | 28.11 | |||
0.30 | 0.80 b | 1.30 b | 1.70 b | 68.55 b | ||||||||
0.50 | 0.54 c | 0.66 c | 0.80 c | 59.51 c | ||||||||
6-BA | 0 | 0.78 b | 0.17 | 1.56 a | 0.43 | 1.92 b | 0.56 | 76.76 a | 8.44 | |||
0.15 | 0.81 a | 1.55 a | 2.13 a | 70.59 b | ||||||||
0.30 | 0.64 c | 1.13 b | 1.57 c | 68.32 b | ||||||||
ABA | 0 | 0.71 c | 0.07 | 1.31 c | 0.23 | 1.77 b | 0.19 | 71.57 ab | 3.63 | |||
0.50 | 0.74 b | 1.54 a | 1.96 a | 73.87 a | ||||||||
1.00 | 0.78 a | 1.40 b | 1.88 a | 70.24 b |
Table 4
Effect of exogenous hormones on fresh weight and somatic embryogenesis of suspension cells for Larix kaempferi"
激素 Hormone | 浓度 Concentration/(mg·L-1) | 悬浮细胞鲜质量 Fresh weight of suspension cells/g | 体胚发生数量 Number of somatic embryos/(embryo·g-1) | |||||||||
7 d | 14 d | 21 d | 均值 Mean | 极差 Range | ||||||||
均值 Mean | 极差 Range | 均值 Mean | 极差 Range | 均值 Mean | 极差 Range | |||||||
2, 4-D | 0.15 | 4.02 a | 0.42 | 5.25 a | 1.14 | 7.80 a | 3.07 | 87.31 a | 13.08 | |||
0.30 | 3.60 b | 4.85 a | 7.48 b | 83.65 b | ||||||||
0.50 | 3.92 a | 4.11 b | 4.73 c | 74.23 c | ||||||||
6-BA | 0 | 3.86 b | 0.41 | 4.79 ab | 0.82 | 6.73 a | 0.31 | 82.09 b | 11.40 | |||
0.15 | 3.41 c | 4.31 b | 6.86 a | 75.85 c | ||||||||
0.30 | 4.27 a | 5.13 a | 6.42 a | 87.25 a |
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