Scientia Silvae Sinicae ›› 2020, Vol. 56 ›› Issue (10): 53-62.doi: 10.11707/j.1001-7488.20201006
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Weibo Sun,Zhaoqiong Wei,Xiaoxing Ma,Hui Wei,Qiang Zhuge*
Received:
2019-11-14
Online:
2020-10-25
Published:
2020-11-26
Contact:
Qiang Zhuge
CLC Number:
Weibo Sun,Zhaoqiong Wei,Xiaoxing Ma,Hui Wei,Qiang Zhuge. Safety Assessment of a Field Trial of Three Types of Transgenic Poplar Nanlin895[J]. Scientia Silvae Sinicae, 2020, 56(10): 53-62.
Fig.1
Planting map of experimental field A1-3, A3-4, A4-6, A5-0, A5-23: Bt insect-resistant lines of transgenic Bt toxin protein coding gene (Cry1Ac); TLP1-2, TLP1-6, TLP1-7, TLP2-9, TLP2-11: TLP pathogen-resistant lines of transgenic thaumatin-like protein coding gene (PeTLP); Gols2-1, Gols2-2, Gols2-3: Gols stress-resistant lines of transgenic AtGols gene; NL895: Non-transgenic Nanlin895 poplars. "
Table 1
PCR primers, annealing temperature(Tm) and amplification fragment size for detection of transgenic poplar"
目的基因 Target gene | 引物序列 Primer sequence | 退火温度 Tm/℃ | 目的片段 Target fragment/bp |
Cry1Ac | F: 5′-CTATCCTTCGCAAGACCCTTC-3′ R: 5′-ACAACTGCGATAAATACCAC-3′ | 56 | 1 977 |
PeTLP | F: 5′-CTATCCTTCGCAAGACCCTTC-3′ R: 5′-CTTTGAAAGGAAACTGCAGGGA-3′ | 56 | 890 |
AtGols | F: 5′-CTATCCTTCGCAAGACCCTTC-3′ R: 5′-GGCCGTGAACAAAATAACTC-3′ | 58 | 1 131 |
Table 2
PCR primers, annealing temperature(Tm) and amplification fragment size for detection of soil microorganisms"
目的基因 Target gene | 引物序列 Primer sequence | 退火温度 Tm/℃ | 目的片段 Target fragment/bp |
Cry1Ac | F: 5′-GCTCCGGGGCTTACTCAAAC-3′ R: 5′-ACAACTGCGATAAATACCAC-3′ | 56 | 1 862 |
PeTLP | F: 5′-CATTTCGGTGAAAGGAAAAGAG-3′ R: 5′-CGGGATCCCTAGTGACAGAAGATAAC-3′ | 56 | 775 |
AtGols | F: 5′-GCTCCGGGGCTTACTCAAAC-3′ R: 5′-GGCCGTGAACAAAATAACTC-3′ | 58 | 1 028 |
Table 3
Effect of transgenic poplars on growth of lettuce seed"
样品编号 Sample code | 胚根长度 Length of radicle/cm | 下胚轴长度 Length of hypocotyl/cm | |||||||
2018-04 | 2018-06 | 2018-08 | 2018-10 | 2018-04 | 2018-06 | 2018-08 | 2018-10 | ||
CK- | 1.26±0.18 | 1.65±0.21 | 1.34±0.13 | 1.94±0.22 | 0.98±0.06 | 1.32±0.18 | 1.14±0.08 | 0.86±0.12 | |
NL895 | 0.71±0.14 | 0.81±0.05 | 0.69±0.12 | 0.88±0.36 | 0.80±0.12 | 0.92±0.12 | 0.94±0.15 | 0.61±0.12 | |
TLP1-2 | 0.63±0.11 | 0.69±0.13 | 0.53±0.09 | 0.83±0.28 | 0.89±0.10 | 0.88±0.11 | 0.86±0.19 | 0.53±0.14 | |
TLP1-6 | 0.54±0.12 | 1.11±0.18 | 0.46±0.17 | 0.91±0.21 | 0.67±0.08 | 1.12±0.09 | 0.56±0.15 | 0.65±0.12 | |
TLP1-7 | 0.69±0.15 | 0.74±0.31 | 0.43±0.17 | 0.87±0.31 | 0.67±0.12 | 0.82±0.08 | 0.81±0.14 | 0.63±0.05 | |
TLP2-9 | 0.83±0.18 | 0.63±0.10 | 0.63±0.17 | 0.72±0.25 | 0.73±0.08 | 1.11±0.13 | 0.74±0.15 | 0.66±0.13 | |
TLP2-11 | 0.72±0.13 | 0.92±0.14 | 0.71±0.21 | 0.82±0.13 | 0.69±0.12 | 0.96±0.10 | 0.88±0.09 | 0.46±0.08 | |
A1-3 | 0.53±0.09 | 0.83±0.28 | 0.98±0.15 | 0.84±0.09 | 0.86±0.13 | 0.53±0.11 | 0.79±0.08 | 0.85±0.12 | |
A3-4 | 0.98±0.17 | 0.46±0.21 | 0.88±0.31 | 0.81±0.09 | 0.99±0.14 | 0.56±0.05 | 0.62±0.13 | 0.92±0.13 | |
A4-6 | 0.52±0.31 | 0.87±0.17 | 0.72±0.15 | 0.63±0.22 | 0.90±0.17 | 0.63±0.25 | 0.81±0.12 | 0.92±0.18 | |
A5-0 | 0.63±0.12 | 0.73±0.17 | 0.64±0.25 | 0.76±0.12 | 1.09±0.28 | 0.63±0.15 | 0.73±0.31 | 0.72±0.15 | |
Glos2-1 | 0.65±0.12 | 0.89±0.36 | 0.81±0.14 | 0.43±0.13 | 0.69±0.17 | 0.66±0.12 | 0.92±0.33 | 0.52±0.13 | |
Glos2-2 | 0.87±0.15 | 0.82±0.13 | 0.80±0.13 | 0.93±0.07 | 0.79±0.12 | 0.46±0.08 | 0.83±0.28 | 0.97±0.15 | |
Glos2-3 | 0.92±0.14 | 0.84±0.12 | 0.69±0.18 | 0.71±0.13 | 0.83±0.25 | 0.85±0.18 | 0.88±0.12 | 0.66±0.15 |
Table 4
Statistical analysis of the effect of transgenic poplars on lettuce seed growth"
株系 Line | 差异源 Source of difference | 胚根生长统计数据 Statistical data of radicle growth | 下胚轴生长统计数据 Statistical data of hypocotyl growth | |||||||||||
平方和 SS | 自由度 Df | 均方 MS | F | P | 临界值 Critical value | 平方和 SS | 自由度 Df | 均方 MS | F | P | 临界值 Critical value | |||
TLP | 外源基因 Exogenous gene | 0.051 8 | 5 | 0.010 4 | 0.642 7 | 0.671 1 | 4.555 6 | 0.025 8 | 5 | 0.005 2 | 0.368 4 | 0.862 3 | 4.555 6 | |
采样时间 Sampling date | 0.270 9 | 3 | 0.090 3 | 5.603 3 | 0.008 8 | 5.417 0 | 0.439 5 | 3 | 0.146 5 | 10.471 4 | 0.000 6 | 5.417 0 | ||
误差 Error | 0.241 7 | 15 | 0.016 1 | 0.209 9 | 15 | 0.014 0 | ||||||||
总计 Total | 0.564 4 | 23 | 0.675 2 | 23 | ||||||||||
Bt | 外源基因 Exogenous gene | 0.045 2 | 4 | 0.011 3 | 0.419 1 | 0.791 8 | 5.412 0 | 0.011 0 | 4 | 0.002 7 | 0.124 1 | 0.971 0 | 5.412 0 | |
采样时间 Sampling date | 0.039 8 | 3 | 0.013 3 | 0.492 4 | 0.694 2 | 5.952 6 | 0.189 3 | 3 | 0.063 1 | 2.854 0 | 0.081 7 | 5.952 6 | ||
误差 Error | 0.323 1 | 12 | 0.026 9 | 0.265 4 | 12 | 0.022 1 | ||||||||
总计 Total | 0.408 1 | 19 | 0.465 8 | 19 | ||||||||||
Gols | 外源基因 Exogenous gene | 0.052 0 | 3 | 0.017 3 | 0.933 3 | 0.463 8 | 6.991 9 | 0.035 2 | 3 | 0.011 7 | 0.467 6 | 0.712 1 | 6.991 9 | |
采样时间 Sampling date | 0.026 0 | 3 | 0.008 7 | 0.467 2 | 0.712 3 | 6.991 9 | 0.094 9 | 3 | 0.031 6 | 1.261 2 | 0.344 7 | 6.991 9 | ||
误差 Error | 0.167 1 | 9 | 0.018 6 | 0.225 7 | 9 | 0.025 1 | ||||||||
总计 Total | 0.245 0 | 15 | 0.355 7 | 15 |
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