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Scientia Silvae Sinicae ›› 2016, Vol. 52 ›› Issue (5): 126-133.doi: 10.11707/j.1001-7488.201605015

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Influence of Burkholderia pyrrocinia JK-SH007 on the Microbial Population and Functional Diversity of Microbial Communities in the Rhizosphere Soil of Poplar

Ren Jiahong1,2, Li Hao1, Liu Hui1, Ye Jianren1, Wu Xiaoqin1   

  1. 1. College of Forestry, Nanjing Forestry University Nanjing 210037;
    2. Department of Biological Science and Technology, Changzhi College Changzhi 046011
  • Received:2014-10-13 Revised:2015-10-12 Online:2016-05-25 Published:2016-06-01

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Abstract:

[Objective] Burkholderia pyrrocinia JK-SH007 is a strain of biocontrol bacteria which was isolated from the stem of poplar. In order to evaluate the safety of B. pyrrocinia JK-SH007, this study aimed to evaluate the micro ecological effects and the biological safety of the natural environment after its entry into the natural environment. [Method] A solid plate method was used to investigate the potential effects of JK-SH007 on the activity change of the four main soil enzymes, and the Biolog ECO microplate method was applied to assay the microbial community and its function diversity in rhizosphere soil of aigeiros poplar (Populus deltoides). [Result] The results showed that from the over trend, the numbers of bacteria, actinomyces and fungi in poplar rhizosphere soil gradually increased and were significantly higher than control with the extension of time after being inoculated with strain JK-SH007. The number of these microorganisms reached the peak (3.36×109 cfu·g-1 dry soil; 7.50×107 cfu·g-1 dry soil; 1.14×107 cfu·g-1 dry soil) in 150 day post-inoculation, and then decreased significantly, but by then were still higher than these of control plants. The soil enzymes activities (acid phosphatase, alkaline phosphatase, dehydrogease and invertase) with inoculation of strain JK-SH007 were all higher than that of control, especially for alkaline phosphatase and invertase. The Biolog ECO plates analysis showed that AWCD values on day 10, day 20, day 60, day 150 and day 180 after inoculation were higher than control treatment. At the early stage of the inoculation, soil microbial diversity (Shannon index, Simpson index and McIntosh index) had no obvious regularity. At the 120th day, 150th day and 180th day post-inoculation, the three parameters of the functional diversity of the soil microbial communities were all slightly higher than those of control. But, the differences were not significant. [Conclusion] Strain JK-SH007 may play roles in improving total microbial activity of poplar rhizosphere soil, increasing soil fertility and enriching soil communities. All of these are conductive to maintaining and promoting the soil fertility and health. But these benefits would be decline over time. At the same time, all of results showed strain JK-SH007 had no or less menace to the indigenous microorganisms in the environment, suggesting that it is safe to put strain JK-SH007 into use as a potential bio-control factor in the future.

Key words: Populus deltoides, Burkholderia pyrrocinia, microbial population, safety assessment

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