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Scientia Silvae Sinicae ›› 2015, Vol. 51 ›› Issue (11): 69-75.doi: 10.11707/j.1001-7488.20151109

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Effects of Multi-Gene Transgenic Populus×euramericana 'Guariento' on the Function of Microbial Population in the Rhizosphere Soil

Zhu Wenxu, Zhang Bingyu, Huang Qinjun, Chu Yanguang, Ding Changjun, Zhang Weixi, Su Xiaohua   

  1. State Key Laboratory of Tree Genetics and Breeding Research Institute of Forestry, CAF Beijing 100091
  • Received:2014-04-10 Revised:2014-06-06 Online:2015-11-25 Published:2015-12-08

Abstract: [Objective]This paper aims at studying the carbon utilization level of the rhizosphere microbials of transgenic poplars (Populus×euramericana 'Guariento') and non-transgenic poplars, and the results would be helpful in the prediction whether the transgenic poplars have significant adverse effects on the soil microorganism system. [Method]The rhizosphere soil was collected from three different lands:land planted with five strains of transgenic poplars (D5-9,D5-19,D5-20,D5-21 and D5-24), land planted with non-transgenic poplars (CK) and land without any plants(NP) at Hancunhe Dongying nursery in Fangshan District. The ecological plate culture method was used to cultivate soil microbes, multifunction microtiter plate reader was used to calculate the carbon utilization, and the collected data were analyzed with Shannon index, Simpson index and Mclntosh index. [Results]After 24 h incubation, soil microbes gradually adapted to the ecological plate environment, and then entered the logarithmic growth phase, and the arerage well color development (AWCD) rapidly increased until 96 h. A strong microbial activity was observed at this time, and carbon sources began to be obviously used, and then followed by slow growth until stabilized. At each culture stage, the AWCD of D5-24 rhizosphere soil was highest, showing that the amount of carbon source used in the microbial community was the most, and the metabolic activity was the strongest. The non-transgenic rhizosphere was second; and the NP soil microbial had the slowest metabolism and the weakest activity. The result indicated that the rhizosphere soil of poplars was rich in carbon sources, and hence promoted metabolic activity of microorganisms. There were differences in utilization on amines, phenolic acids, carboxylic acids, polymers, amino acids and carbohydrates by soil microorganisms. The carbohydrates and amino acid metabolism were relatively strong while acids and amines utilization was weak. The principal component analysis(PCA) for 72 h data indicated that contribution rate of the first 20 factors was 100% in the 31 principal components of variance. The first main component of a variance had 42.31% contribution rate, the principal component 2 had contribution rate of 13.57 percent, and the third to 20th principal components had smaller contribution, with 1.32%-11.64%. Cumulative contribution rate from PC1 and PC2 was up to 55.88%. Correlation analysis showed that D-cellobiose, the carbon source, had the highest correlation with PC1(0.900), and the carbon source that formed a negative correlation with PC1 was itaconic acid (-0.266). The carbon source that formed the highest positive correlation with PC2 was phenylethylamine (0.668), and the carbon source formed a negative correlation was α-D acid (-0.631). NP and CK were distributed in the minimum and maximum ends, respectively. In the PC2 axis, NP and D5-9 were located in the positive, D5-20 and D5-24 were distributed in the negative, and CK, D5-19 and D5-21 were distributed on both sides. There were significant differences in distribution regions between NP and other clones. [Conclusion]The multi-gene transgenic poplar was not found having significant adverse effects on the soil microorganism system. Differences between genetically clones could be due to the inserted position of exogenous genes, copy number and other differences which lead to changes in physiological and biochemical characteristics of plants and metabolism, not due to the effect of gene expression product itself. Regardless of planting non-transgenic poplar or transgenic poplars can increase the metabolic activity of rhizosphere soil microbes.

Key words: multi-gene transgenic poplar, soil microorganism, Biolog

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