高温与干旱胁迫对‘南林895杨’扦插苗生长和超微结构的影响

doi:10.11707/j.1001-7488.20200520

Abstract

Abstract:

Objective: In this study,we investigated the response of the growth and cell ultrastructure of poplar cutting seedlings to high temperature,drought and their combined stresses,in order to explore the relationship between leaf morphology,growth and ultrastructure damage under stress and thereby reveal the differences of the response mechanism of poplar to different stresses from morphological and cytological perspectives. Methods: Cuttings of Populus×euramericana 'Nanlin-895' poplar were used as the experimental material. Different temperature and moisture gradients were set to study the growth and morphological indexes of poplar under single factor stress and combined stress of high temperature and drought,and the effects of different treatments on cell ultrastructure were investigated under TEM. Results: 1) Compared to the control,the basal diameter,seedling height,biomass and partitioning index of leaves decreased while leaf perimeter to area ratio,the ratio of root to shoot,partitioning indices of root increased under single or combined stress of high temperature and drought. The effects of combined stress on growth morphological index were greater than those of the single stress. 2)Ultrastructure of leaves was damaged to different degrees under single or combined stress of high temperature and drought. Chloroplast swelled,and lamella structure expanded or even melt,osmiophilic granules content increased and starch granule content decreased,and even the arrangement of mesophyll cells was disorganized with damaged membrane structure under severe stress. The damage intensity of leaf ultrastructure was consistent with the stress level. Severe high temperature stress produced more obvious impact on ultrastructure than drought stress. The effects of combined stress on ultrastructure were greater than those of single stress. Conclusion: Under high temperature and drought stress,the damage of ultrastructure of leaves affects the photosynthesis and the stability of the intracellular environment. Normal physiological processes are affected,and eventually results in the changes of seedling growth morphology.In this study,destruction of cell ultrastructure was consistent with the performances of growth morphology,which indicates the feasibility of revealing the response mechanism of plants to stress by cytology and morphology indicator analysis.

Key words: elevated temperature, drought, Populus×euramericana 'Nanlin-895' seedlings, growth morphology, leaf ultrastructure

CLC Number:

Jiajun Yang,Yongbo Wu,Yanhong Zhang. Effects of High Temperature and Drought Stresses on the Growth and Ultrastructure of Populus×euramericana 'Nanlin-895' Cutting Seedlings[J]. Scientia Silvae Sinicae, 2020, 56(5): 176-183.

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References 0

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温度 Temperature/℃	田间持水量 Field capacity(%)	处理 Treatment	株高 Height/cm	地径 Diameter/cm	叶缘分裂程度 Leaf perimeter/area
25	70	T₀D₀	102±5.47a	1.07±0.1a	0.39±0.01c
	55	T₀D₁	81.33±2.71b	0.83±0.02b	0.44±0.02c
	40	T₀D₂	73.47±2.59bc	0.79±0.07b	0.52±0.02bc
35	70	T₁D₀	95.8±5.49a	1.03±0.12a	0.42±0.01c
	55	T₁D₁	72.27±2.45c	0.76±0.02b	0.58±0.03a
	40	T₁D₂	62.8±4.63de	0.73±0.04b	0.54±0.02ab
40	70	T₂D₀	70.27±2.97cd	0.81±0.09b	0.58±0.04a
	55	T₂D₁	60.8±2.12e	0.77±0.08b	0.51±0.01bc
	40	T₂D₂	58.93±3.69e	0.68±0.04b	0.43±0.03c

温度 Temperature/℃	田间持水量 Field capacity(%)	地上部分 Aboveground part/g	茎 Stem/g	叶 Leaf/g	根 Root/g	总生物量 Total biomass/g	根茎比 Root to shoot ratio
25	70	60.39±3.22a	43.03±1.49a	17.37±1.49a	18.71±0.37a	79.10±2.98a	0.31±0.02 d
	55	43.80±3.05bc	31.33±3.63bc	12.47±3.63ab	16.34±0.82ab	60.14±2.48bc	0.38±0.04cd
	40	34.78±1.82cd	27.25±0.31cd	7.53±0.31bcd	14.26±1.07bc	49.04±2.68cd	0.41±0.02bc
35	70	51.40±6.4ab	39.11±4.75ab	12.29±4.75abc	16.97±3.00ab	68.37±9.4ab	0.33±0.02d
	55	43.16±6.52bc	31.55±2.58bc	11.61±2.58abc	15.50±1.25ab	58.65±7.73bc	0.36±0.03cd
	40	35.05±7.2cd	25.70±3.23cd	9.36±3.23bcd	15.90±1.4ab	50.95±8.48cd	0.46±0.06ab
40	70	26.54±5.24de	20.37±1.7de	6.17±1.7cd	11.34±1.82cd	37.89±7.06de	0.43±0.01bc
	55	21.92±4.26e	16.52±2.02e	5.40±2.02d	10.16±2.33d	32.08±6.59e	0.46±0.02ab
	40	19.46±3.08e	15.19±0.93e	4.26±0.93d	9.84±1.32d	29.30±4.29e	0.51±0.04a

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Effects of High Temperature and Drought Stresses on the Growth and Ultrastructure of Populus×euramericana 'Nanlin-895' Cutting Seedlings

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