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Scientia Silvae Sinicae ›› 2018, Vol. 54 ›› Issue (9): 44-48.doi: 10.11707/j.1001-7488.20180906

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Ploidy Study on Polyploidy Cultivars of Camellia reticulata

Xu Xiaodan1, Shao Weizhu2, Zheng Wei3   

  1. 1. Faculty of Art and Communication, Kunming University of Science and Technology Kunming 650504;
    2. Management Station of Camellia Base, Tengchong Bureau of Housing and Urban-Rural Development Tengchong 679101;
    3. Faculty of Architecture and City Planning, Kunming University of Science and Technology Kunming 650504
  • Received:2017-10-31 Revised:2017-11-15 Online:2018-09-25 Published:2018-09-10

Abstract: [Objective] The paper was intended to clarify the ploidy of main cultivars of Camellia reticulata, which will be helpful for the breeding in the future.[Method] Leave samples of 63 cultivars of C. reticulata as well as the diploid species C. saluenensis used as control were collected from Kunming Botanical Garden, and then DNA was extracted and contents were detected using fluorescence intensity by flow cytometry (FCM). The ploidy were estimated according to their 2C values. Additionally, the chromosome numbers were counted by electron microscope using stem tips and pollen of three representative cultivars in each ploidy group, so as to verify the result of flow cytometry.[Result] Among the 63 cultivars of C. reticulata, the maximum DNA content was of ‘Hentiangao’ which was peaking at 304.43 with 2C value of 18.32 pg. The minimum DNA content was of ‘Hongxia Yingchun’ which was peaking at 175.03 with 2C value of 10.53 pg. A total of 29 cultivars (46.03% of tested cultivars) which had DNA peaking below 250 with 2C value below 15.00 pg were estimated as hexaploid. 31 cultivars (49.21%), which had DNA peaking range of 250 to 300 and 2C value of 15.00-18.00 pg, were estimated to be octaploid. Three cultivars (4.76%) which had DNA peaking above 300 and 2C value above 18.00 pg were estimated as decaploid. Furthermore, the chromosome numbers of representative cultivars, ‘Wujiao Xiuqiu’ (pollen:n=45), ‘Juban’ (pollen:n=60) and ‘Hentiangao’ (stem tip:2n=150), were coincident to flow cytometry result.[Conclusion] The cultivars of C. reticulata exhibited hexaploidy, octoploidy and decaploidy. The abundant cell ploidy would provide a significant basis for the further studies on inheritance and evolution of polyploidy, as well as parents selection for cross breeding.

Key words: Camellia reticulata, cultivar, polyploidization, hybridization, flow cytometry

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