Welcome to visit Scientia Silvae Sinicae，Today is

Scientia Silvae Sinicae ›› 2014, Vol. 50 ›› Issue (4): 121-128.doi: 10.11707/j.1001-7488.20140418

Previous Articles     Next Articles

Change of Endogenous Plant Hormones Contents during Seedling Growth of Picea schrenkiana Treated with DHAP

Zhang Jianhong1, Chen Zhiying2, Ruan Xiao2, Zhang Yuzhu2, Pan Cunde3, Wang Qiang2   

  1. 1. Ningbo Agriculture Academy Ningbo 315040;
    2. Ningbo Institute of Technology, Zhejiang University Ningbo 315100;
    3. College of Forestry and Horticulture, Xinjiang Agricultural University Urumqi 830052
  • Received:2013-10-12 Revised:2014-02-17 Online:2014-04-25 Published:2014-05-06
  • Contact: 王强

PDF (PC)

721

Abstract: Schrenk spruce (Picea schrenkiana), a major tree species in the forest ecosystems of Xinjiang, plays an important role in water conservation. 3,4-dihydroxyacetophenone (DHAP) is a principal autotoxic substance in P. schrenkiana needles as well as its litter, and the substance may contribute to P. schrenkiana natural regeneration. In this paper, we determined the change of endogenous plant hormones (ZT、GA3、IAA and ABA) contents during seedling growth of P. schrenkiana with the ultra performance liquid chromatography (UPLC), and measured growth in radicle, plumule and fresh weight of the seedlings subjected to DHAP treatments, trying to explain physiological mechanisms of the autotoxic effects. The results showed that DHAP treatment had no significant effect on plumule growth of P. schrenkiana but had significant inhibitory effect on fresh weight (The fresh weight treated with 0.1 mmol·L-1 DHAP significantly increased in 10 d). The impact of DHAP treatment on radicle elongation had a concentration effect in an order of: 5 mmol·L-1>1 mmol·L-1>0.1 mmol·L-1>control (After 10 d, radicle elongation of 0.1 mmol·L-1 DHAP treatment groups increased significantly). With DHAP concentration increased, the GA3 concentration decreased, the ABA concentration increased, and the ratios of ABA/ZT、ABA/IAA、ABA/(ZT+GA3+IAA) increased. In 4-6 d, after 5 mmol·L-1 DHAP treatment, the IAA concentration increased greatly (1.24-1.70 times higher than that of control group), which might inhibit the seedling growth. For 0.1 mmol·L-1 DHAP treatment group, after 10 d, the concentration of ZT and IAA increased, the concentration of ABA decreased, and the ratios of ABA/ZT、ABA/IAA、ABA/(ZT+GA3+IAA) decreased, which might cause the radicle elongation and fresh weight increasing rapidly. It is suggested that the change of contents and the ratios of endogenous plant hormones during the seedling growth caused by DHAP treatment might be the key factor inhibiting (promoting) or delaying the seedling growth (radicle, plumule elongation and fresh weight) of P. schrenkiana.

Key words: Picea schrenkiana, DHAP, UPLC, seedling growth, endogenous plant hormones

CLC Number: