基于分子可操作分类单元的北京地区杨树内生葡萄座腔菌属真菌多样性

doi:10.11707/j.1001-7488.20140117

Abstract

Abstract:

Diversity of endophytic fungi in trees is a key to tree health and interaction between trees and environment. Operational taxonomic units based on molecular DNA barconding an important role in disclosing microbiological diversity. Based on criteria and techniques of DNA barcoding approaches, a validation database on ITS sequences of Botryosphaeria genus and its related fungi downloaded from public nucleotide databases in NCBI, was constructed for determining the strains from endophytes in poplars in Beijing area, along with their ITS amplification and phylogenetic relationship analysis. The results showed that there were three populations of Botryosphaeria species parasitic in poplars as endophytes,B. dothidea, B. rhodina and B. parva. Strains of each population had genetica diversity. The approaches and results, presented in this paper,would contribute to a comprehensive understanding endophyte diversity in poplars and promoting researches on relationships between poplar hosts and Botryosphaeria pathogen as well as poplar canker fungal pathagenesis and control.

Key words: endophyte, Botryosphaeria, DNA barcoding, classification, diversity

CLC Number:

S718.81

Li Ruhua, Yan Donghui, Feng Xiaohui, Sun Xiaoming. Diversity of Botryosphaeria spp., as Endophytes in Poplars in Beijing, Based on Molecular Operational Taxonomic Units[J]. Scientia Silvae Sinicae, 2014, 50(1): 109-115.

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Diversity of Botryosphaeria spp., as Endophytes in Poplars in Beijing, Based on Molecular Operational Taxonomic Units

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