青杄PwPSAF基因的克隆与组织表达分析

doi:10.11707/j.1001-7488.20131007

Abstract

Abstract:

PSAF is F subunit of photosystem Ⅰ reaction center complex in higher plants and plays an important role during the process of electron mobility of photosynthesis. The full length cDNA of PwPSAF was obtained by RACE PCR assays based on the cDNA library of Picea wilsonii and EST fragment of PwPSAF. Several pieces of software and tools such as Expasy, DNAMAN, ClustalX and MEGA were used to analyze the physical and chemical properties and secondary and tertiary structures of PwPSAF. Expression level of PwPSAF in different tissues of seedlings was identified by RT-qPCR under drought and salt treatments. Furthermore, the expression patterns were investigated at different stages of seed germination. We found that PwPSAF was composed of 253 amino acids and the theoretical molecular weight was 27.04 kDa with isoelectric point of 9.57. The protein has two transmembrane domains and shares conserved C domain with other species such as Picea sitchensis and Vitis vinifera. RT-qPCR assays indicated that PwPSAF was mainly expressed in needles of Picea wilsonii. During seed germination, PwPSAF showed the highest expression within 2 days after germination, and then declined on the fourth day. With the cotyledons grown, the expression of PwPSAF was up-regulated again. In addition, the expression level of PwPSAF was down-regulated in the needles under drought and salt stresses. These results suggest that PwPSAF in P. wilsonii plays an important role during seed germination and in response to abiotic stresses.

Key words: Picea wilsonii, PSAF, bioinformatic analysis, tissue-specific expression, stress response

CLC Number:

S718.46

Li Changjiang, Sun Fan, Zhang Tong, Zhang Lingyun. Cloning and Tissue Expression Analysis of PwPSAF in Picea wilsonii[J]. Scientia Silvae Sinicae, 2013, 49(10): 40-47.

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Cloning and Tissue Expression Analysis of PwPSAF in Picea wilsonii

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