Abstract:

Tissue culture of young and mature bamboo of Dendrocalamus sinicus was studied systematically from aspects of explant collection, disposal, in vitro; and shoot inducement, proliferation, rooting; and plantlet hardening and so on in this experiment. The best media for every stage were discussed by orthogonal experiment. The results showed that the dosage of hormone BA and the shoot multiplication were proportioned positively. But if the dosage of BA enlarged or a long time's culture made in high thick of BA, flower buds would be seen. So BA had the function of making cells become progenitive performance from nutritious performance. Hormone KT was the main factor in height growth of shoot multiplication, which could make nodes elongate, make buds grow well and make cells become nutritious performance from progenitive performance. Coconut water (CW) had function of enhancing growth. Hormone IBA was the main factor in rooting. A few hormone NAA in combination with IBA could make rooting well and growing well. And the plantlets showed highly survival rate in hardening. The successes of explants in vitro and induced multiplication of shoots were related to the collected time and collected section etc. of the explants.

Key words: Dendrocalamus sinicus, tissue culture, in vitro rapid propagation, embryos, mature bamboo