Abstract:

This paper reported that an in vitro embryogenic callus induction and plantlet regeneration system from immature zygotic embryos of hybrid larch was established. Embryogenic calli were induced from zygotic embryos of Larix kaempferi 5×L. olgensis 77-3 and Larix kaempferi 5×L. gmelinii 9. The embryogenic callus induction rate was the highest when zygotic embryos were collected 65-days after pollination and cultured on S medium supplemented with 0.5 mg·L^-1 2，4-D +　0.5 mg·L^-1 6-BA + 0.5 mg·L^-1 KT， with 10% embryogenic callus induction rate. Three embryogenic cell lines were found to have stable regenerating ability. The cell lines from zygotic embryos of L.kaempferi 5×L.olgensis 77-3 collected 65-days after pollination were the best， with 18.1，77.0%, and 28.1% of number of somatic embryo per gram embryogenic callus， germination rate and plant regeneration rate， respectively. Up to 41.8% of the transplanted plantlets were successfully survived in soil.

Key words: hybrid larch, embryogenic callus, zygotic embryo, tissue culture