毛白杨纤维素合酶基因(PtoCesA1)的克隆及其在烟草中的遗传转化

doi:10.11707/j.1001-7488.20070307

Scientia Silvae Sinicae ›› 2007, Vol. 43 ›› Issue (03): 39-45.doi: 10.11707/j.1001-7488.20070307

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Isolation and Transformation in Nicotiana tabacum of a Cellulose Synthase Gene (PtoCesA1) from Populus tomentosa

Li Chunxiu,Qi Liwang,Shi Shengqing,Wang Yangdong,Wang Jianhua,Zhang Shougong

Laboratory of Cell Biology, Research Institute of Forestry, CAF Beijing 100091

Received:2005-12-09 Revised:1900-01-01 Online:2007-03-25 Published:2007-03-25

Abstract

Abstract:

A cellulose synthase gene(PtoCesA1), isolated from Populus tomentosa, was 3 215 bp long with an opening reading frame of 2 937 bp extending from nucleotides 52～2 988. Comprison of the nucleotide sequence of PtoCesA1 with PtrcesA1 from Populus tomentosa showed 97% identity. The full length PtoCesA1 cDNA was subcloned into pBI121 and identified by digestion, then transformed into Nicotiana tabacum by leaf disc. The transgenic plants with PtoCesA1 had small leaves, and “dwarf” phenotype,consistent with a loss of xylem and fiber cell wall thickness。

Key words: Populus tomentosa, PtoCesA1, transformation

Li Chunxiu;Qi Liwang;Shi Shengqing;Wang Yangdong;Wang Jianhua;Zhang Shougong. Isolation and Transformation in Nicotiana tabacum of a Cellulose Synthase Gene (PtoCesA1) from Populus tomentosa[J]. Scientia Silvae Sinicae, 2007, 43(03): 39-45.

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Isolation and Transformation in Nicotiana tabacum of a Cellulose Synthase Gene (PtoCesA1) from Populus tomentosa

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