过量表达LAR3和Bbchit1以提高毛白杨对叶枯病的抗性

doi:10.11707/j.1001-7488.20120715

Scientia Silvae Sinicae ›› 2012, Vol. 48 ›› Issue (7): 92-97.doi: 10.11707/j.1001-7488.20120715

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Overexpression of LAR3 and Bbchit1 Genes in Transgenic Populus tomentosa Raising the resistance to Alternaria alternata

Huang Yan, Liu Hong, Yuan Li, Jia Zhichun, Luo Keming

Institute of Resources Botany of Southwest University Key Laboratory of Eco-Environments of Three Gorges Reservoir Region of Ministry of Education School of Life Sciences, Southwest University Chongqing 400715

Received:2011-07-23 Revised:2011-12-21 Online:2012-07-25 Published:2012-07-25

Abstract

Abstract:

Plant genetic engineering offers an alternative opportunity to enhance plant resistance to fungal pathogens. In this study, a chitinase gene (Bbchit 1 ) from Beauveria bassiana was introduced into transgenic plants of Chinese white poplar (Populus tomentosa) which constitutively overexpresses LAR 3 via Agrobacterium-mediated retransformation. The expression of Bbchit 1 and LAR 3 was detected in transgenic plants by real time-PCR. In vivo assays demonstrated that the transgenic poplar plants displayed a significant reduction in their disease symptoms after infected with Alternaria alternata compared to control plants. These results suggest that constitutive expression of LAR 3 and Bbchit 1 genes can be exploited to improve resistance to fungal pathogens in the poplar.

Key words: LAR 3, Bbchit 1, retransformation, Populus tomentosa, Alternaria alternata

CLC Number:

S763.1

Huang Yan;Liu Hong;Yuan Li;Jia Zhichun;Luo Keming. Overexpression of LAR3 and Bbchit1 Genes in Transgenic Populus tomentosa Raising the resistance to Alternaria alternata[J]. Scientia Silvae Sinicae, 2012, 48(7): 92-97.

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Overexpression of LAR3 and Bbchit1 Genes in Transgenic Populus tomentosa Raising the resistance to Alternaria alternata

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