竹子捕光叶绿素a/b结合蛋白基因全长的克隆和序列分析

doi:10.11707/j.1001-7488.20070306

Scientia Silvae Sinicae ›› 2007, Vol. 43 ›› Issue (03): 34-38.doi: 10.11707/j.1001-7488.20070306

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Cloning and Characterization of a Full-Length Gene Encoding the Light Harvesting Chlorophyll a/b-Binding Protein in Bamboo

Gao Zhimin¹,Li Xueping¹,Peng Zhenhua²,Yue Yongde¹

1. Key Laboratory of Bamboo and Rattan Science and Technology of State Forestry Administration International Center for Bamboo and Rattan Beijing 100102; 2. Research Institute of Forestry, CAF Beijing 100091

Received:2006-11-10 Revised:1900-01-01 Online:2007-03-25 Published:2007-03-25

Abstract

Abstract:

A full-length Cdna of cab gene was cloned from the first strand of bamboo(Bambusa oldhamii) cDNA through RT-PCR and RACE methods, named as cab-BO1 (cab gene from B. oldhamii). The length of cab-BO1(GenBank accession number:EF061137) is 1 102 bp,which contains an open reading frame encoding 265 amino acids from 64 th to 861 th position. The bioinformatics analysis indicated that the protein encoded by cab-BO1 has a chlorophyll a/b binding domain(64^th～²³²th position),a protein kinase C phosphorylation site (9^th～11^th position), a ubiquitin domain signature (27^th～52^th position),and a casein kinase II phosphorylation site(55^th～58 th position). The DNA sequence and encoding amino acid sequence of cab-BO1 showed high similarity with the cab genes of Triticum aestivum,Musa acuminata,Zea mays and Oryza sativa, more than 85% and 89% repectively. The result indicates a clue that cDNA of cab family genes are conserved.

Key words: bamoo(Bambusa oldhamii), light harvesting chlorophyll a/b-binding protein(cab), gene cloning, characterization

Gao Zhimin;Li Xueping;Peng Zhenhua;Yue Yongde. Cloning and Characterization of a Full-Length Gene Encoding the Light Harvesting Chlorophyll a/b-Binding Protein in Bamboo[J]. Scientia Silvae Sinicae, 2007, 43(03): 34-38.

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Cloning and Characterization of a Full-Length Gene Encoding the Light Harvesting Chlorophyll a/b-Binding Protein in Bamboo

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