Abstract:

The sex of anyone of seeds and seedlings of dioecious Ginkgo biloba can not be identified, so we are unable to use their roots as materials to make chromosomes samples according to the traditional method while we study the sex of Ginkgo biloba.Moreover, it isn't feasible to collect roots from mature pistillate and staminate plants.In order to resolve this problem, we used young anthers, leaves, ovules, microspores and female gametophyte in free-nuclear stage of Ginkgo biloba respectively as plant materials and used cycloheximide (100 mg/kg) to treat plant materials alive.We have obtained wonderful chromosomes samples in the end.Pretreatment with cycloheximide is also discussed briefly.

Key words: Ginkgo biloba L., Cycloheximide, Chromosome