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Scientia Silvae Sinicae ›› 1996, Vol. 32 ›› Issue (2): 125-133.

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STUDY ON TISSUE CULTURE OF LARIX OLGENSIS

Kexian Wu1,Wei Li1,Miaozhen Xu1,Changshun Dang2,Yue Liu2   

  1. 1. Heilongjiang Institute of Forestry Harbin 150040
    2. Jiangshanjiao Experimental Forest Station of Heilongjiang Province Mudanjiang 157436
  • Received:1995-01-03 Online:1996-03-25 Published:1996-03-25

Abstract:

Regenerated plantlets used for forestation were induced from axillary buds culture in vitro of plus trees 5-35 years old of Larix olgensis Henry.Z and SH media supplemented with BA1.0 (mg/L, the following units are the same) and NAA0.1 were better for induction of bud differentiation, induction rate of 55% in average and 68% at the most were obtained. Z medium added with IBA0.2, NAA0.2 and Phloroglucinol 40 was better for rooting.It was observed that the basal medium takes notable effect on rooting in this experiment.The differentiation rate of explant would be increased by 25.80% with pretreatment for donor plants. Better procedure of micropropagation for larch should be as follows:selecting plus trees→pretreatment of donor plants→inoculation→inducing differentiation of bud→subculture→rooting→transplanting.

Key words: Larix olgensis, Tissue culture, Differentiation, Rooting