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Scientia Silvae Sinicae ›› 2021, Vol. 57 ›› Issue (8): 102-111.doi: 10.11707/j.1001-7488.20210810

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Analysis of Genetic Diversity and Genetic Structure in Geographic Populations of Corythucha ciliata(Hemiptera: Tingidae) from China Based on Mitochondrial DNA COⅠ Gene Sequences

Yuanchen Zhang1,2,Shaohui Lu3,*,Dongfeng Gong3,Xingli Ma4   

  1. 1. College of Biological and Food Engineering, Anyang Institute of Technology, Henan Province Anyang 455000
    2. Taihang Mountain Forest Pests Observation and Research Station of Henan Province Linzhou 456550
    3. Henan Academy of Forestry Zhengzhou 450002
    4. Center for Integrated Pest Management, College of Agriculture and Life Sciences, North Carolina State University Raleigh 27695
  • Received:2020-11-23 Online:2021-08-25 Published:2021-09-30
  • Contact: Shaohui Lu

Abstract:

Objective: Corythucha ciliata(Hemiptera: Tingidae), an important invasive pest in China, mainly damages on Platanus plants. It has broken out in multiple regions of China. This research tries to explore the genetic differentiation among the geographic population of C.ciliata, and probe its spread path. Method: The sectional nucleotide sequences of COⅠ gene of 240 individuals from 12 geographic populations were determined. Based on their nucleotide sequences, the genetic differentiation, gene flow level, molecular variance, and population demographic history were analyzed by using DnaSP V6.12.03 and other analysis software. Result: There were 13 mutation sites and 9 haplotypes in the COⅠ gene among 12 geographic populations. Among them, 5 haplotypes were shared by all the geographic populations, and the haplotype H1 was an ancestor haplotype. Haplotype diversity index (Hd) of the total population was 0.584, and the Hd value among geographic populations ranged from 0 to 0.758. The fixed coefficient(FST) of the total population was 0.48, and the value between geographic populations ranged from-0.05 to 0.89, suggesting that the degree of differentiation between different populations of C. ciliata was obviously different. Gene flow value(Nm) was 0.55 within the total population and ranged from-85.25 to 47.58 between geographic populations, indicating that the degree of gene exchange was distinct difference among populations. The individual variation within the populations accounted for 52.19% of the total variation, while populations of C. ciliata from the Central China experienced a population expansion event. Conclusion: There are significant differentiation of genetic structures among different groups and different geographical populations of C.ciliata. The genetic differentiation of geographic populations primarily derives from variation of intraspecies. The genetic distance of the geographical populations is no significant association with geographical distance, and the geographical distance has no obvious effect on the gene flow of populations. Furthermore, we speculate that the insect spread from Shanghai as the center to other regions in China.

Key words: Corythucha ciliata, mt DNA COⅠ gene, fixed coefficient, genetic differentiation, gene flows

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