杨树PagABP1介导生长素信号影响植株生长

doi:10.11707/j.1001-7488.LYKX20250329

Abstract

Abstract:

Objective: Through genome-wide identification and bioinformatics analysis of PagABP gene family of Populus alba × P. glandulosa, the gene PagABP1 that is highly expressed in young stems, cambium, xylem and phloem was selected to explore its effect on radial and height growth of this poplar. This study aims to provide a theoretical basis for elucidating how the auxin signaling pathway regulates secondary growth of stems by studying the regulatory role of this gene in the radial and height growth of poplars. Method: Bioinformatics and related software were used to identify the members of the PagABP gene family in 84K poplar. The real-time fluorescence quantitative PCR was used to analyze the expression of PagABP1 gene in terminal buds, young leaves, young stems, young roots, mature stems, old roots, root tips, phloem, vascular cambium and xylem. Plant P_PagABP1::GUS promoter vector and overexpressing 35S::PagABP1 vector were constructed by using the PagABP1 promoter and coding region sequences, respectively. Agrobacterium-mediated method was used to create 84K poplar transgenic materials that were used to identify the effects of PagABP1 gene on poplar radial and height growth. Result: Two ABP homologous genes were identified in 84K poplar genome, and the PagABP1 gene showed relatively higher expression levels in the apical bud, young stems, phloem, cambium, and xylem. The expression locations of P_PagABP1::GUS in transgenic poplar were overlapped with those of P_pagDR5::GUS (auxin response reporter gene), mainly in the terminal bud, young stem, cambium, phloem, and primary xylem, suggesting that PagABP1 is involved in auxin-mediated regulation of radial and height growth. The germination number and growth rate of PagABP1-OE lines were significantly higher than those of the control when the truncated seedlings were planted in soil. Analysis of transgenic lines (#17、#26) that overexpressed PagABP1 gene at 60 days showed that the width of the cambium, primary xylem, and phloem of 12th internode, plant height and basal diameter increased by 50.2%, 43.3%, 1.58%, 19.14% and 19.29%, respectively, compared to the control. Conclusion: PagABP1 gene is associated with auxin expression site, mainly expressed in terminal buds, young stem, phloem, cambium, and primary xylem, affecting radial and height growth. This study can lay a foundation for further revealing the molecular mechanism of PagABP1 gene involved in stem growth of 84K poplar.

Key words: Populus alba × P. glandulosa, auxin signaling, PagABP1, radial growth, height growth

CLC Number:

S718.46

Shen Wang,Zhixin Zeng,Jing Qiao,Yuxin Wang,Ruiqi Zheng,Qi Wang,Hui Wu,Qiqi Zhang,Yang Jiao,Wenbo Shu. PagABP1 in Populus Mediates Auxin Signaling to Influence Plant Growth[J]. Scientia Silvae Sinicae, 2026, 62(2): 126-133.

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References 0

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引物名称 Primer name	序列（5'–3'） Sequences（5'–3'）
PagABP1	AGCGGTGTGCTTGTGCTC
PagABP1	CAAACCTCCCCTTCCATA
PagUBQ	GTTGATTTTTGCTGGGAAGC
PagUBQ	GATCTTGGCCTTCACGTTGT

引物名称 Primer name	引物序列 Primer sequence（5'–3'）
ABP1-Pro-F	TTTAATGGAAACCCGTAGTCT
ABP1-Pro-R	AAATGAGCACAAGCACAC
PMV2-JD-F（菌落鉴定Colony identification）	TATGACCATGATTACGCCAAGC
PMV2-F（同源臂Homologous arm）	TGCATCCAACGCGTTGGGAGCTC
PMV2-R（同源臂Homologous arm）	GCCTTCGCCATTCTAGACTCGAG
ABP1-CDS-F	ATGAGGAGGATGAGCGGTGT
ABP1-CDS-R	TCAAAGTTCATCTTTTAGAGG
2301-JD-R（菌落鉴定Colony identification）	TATGACCATGATTACGAATTCGG
2301-F（同源臂Homologous arm）	AGCTTTCGCGAGCTCGGTACC
2301-R（同源臂Homologous arm）	TCTAGAGGATCCCCGGGTACC

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PagABP1 in Populus Mediates Auxin Signaling to Influence Plant Growth

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