闽楠紫色酸性磷酸酶基因（PAPs）家族分析及3个PAPs在低磷胁迫下的功能

doi:10.11707/j.1001-7488.LYKX20240323

Abstract

Abstract:

Objective: Phoebe bournei is well adapted in red soils with low available phosphorus. Purple acid phosphatases (PAPs) are important acid phosphatases that hydrolyse a wide range of substrates in response to low phosphorus stress to release soluble inorganic phosphorus. This study aims to identify PAPs genes in response to low phosphorus stress and verify the functions in order to explore the response mechanism of P. bournei under low phosphorus stress, which will provide a theoretical basis for cultivating germplasm with efficient phosphorus utilization in P. bournei. Method: Based on the genome data of P. bournei, the PAPs gene family was identified by blastp homology and hmmersearch, and the protein sequences of PbPAPs were compared with those of Arabidopsis thaliana and Picea abies to construct a phylogenetic tree. The photosynthetic fluorescence parameters, inorganic phosphorus content, acid phosphatase (ACP) activity and the expression levels of PbPAPs under different phosphorus contents (0 mmol·L^?1 KH₂PO₄ and 1 mmol·L^?1 KH₂PO₄) in two P. bournei families (Jing'an No.3 and Yifeng No.5) were analyzed, and then three PbPAPs were screened out for the subsequent functional study. Result: A total of 22 PbPAPs genes were identified, all contained Metallophos domains, and were unevenly distributed on 10 chromosomes of P. bournei, with amino acid lengths ranging from 326 to 648 aa. Most of PbPAPs proteins had isoelectric points less than 7, and the exons number ranged from 2 to 13. These PbPAPs harbored two pairs of tandem duplications and four pairs of segmental duplications, and the phylogenetic tree was divided into 3 groups. Under low phosphorus treatments, the elongation of the primary root was inhibited, while the root hair density and the surface area of the root system were increased in the seedlings of P. bournei two families. The net photosynthetic rate and F_v/F_m value were decreased significantly, while the acid phosphatase activity increased. The inorganic phosphorus content of Jing'an No.3 leaves firstly declined and then increased slightly, while the change in Yifeng No.5 was relatively stable. A significant decrease of Pi content was observed in the Jing'an No.3 root after 7 days of treatment, while a significant decrease was firstly observed in the Yifeng No.5 after 21 days of treatment, and the change trends in the later stage of treatments were similar. According to RT-qPCR analysis, low phosphorus induced the gene expression level of PbPAP10a, PbPAP15a and PbPAP26. The three genes of PbPAP10a, PbPAP15a and PbPAP26 were overexpressed in P. bournei roots and A. thaliana by Agrobacterium tumefaciens- and A. rhizocarcinosum-mediated genetic transformation, which resulted in a significant enhancement of ACP activity and a significant increase in Pi content. Thus, overexpression of the PbPAPs gene increased ACP activity and Pi content. Conclusion: It is shown that P. bournei has high tolerance to low phosphorus stress, and Yifeng No.5 is more tolerant to low phosphorus stress than that of Jing'an No.3, which is suitable for forestation in barren forest land or acidic low phosphorus forest land. Overexpression of PbPAPs gene increases ACP activity and Pi content, which would help P. bournei to adapt to the low phosphorus environments.

Key words: Phoebe bournei, purple acid phosphatase, low phosphorus stress, gene family, Pi content

CLC Number:

S722.8

Yiman Zhang,Longjun Lu,Yuting Zhang,Qi Yang,Xiao Han,Zaikang Tong,Junhong Zhang. Analysis of Purple Acid Phosphatases Genes (PAPs) Family in Phoebe bournei and the Functions of Three PAPs under Low Phosphorus Stress[J]. Scientia Silvae Sinicae, 2025, 61(5): 146-160.

Figures/Tables 16

Table 1

Table 2

Table 3

Table 4

Fig.1

Table 5

Fig.2

Fig.3

Fig.4

Fig.5

Fig.6

Fig.7

Fig.8

Fig.9

Fig.10

Fig.11

References 0

	陈　锡, 陈　莹, 刘晓霞, 等. FaGST基因过量表达获得拟南芥转基因植株. 贵州农业科学, 2022, 50 (7): 21- 25. doi: 10.3969/j.issn.1001-3601.2022.07.004
	Chen X, Chen Y, Liu X X, et al. Transgenic Arbidopsis thaliana plants with FaGST gene overexpression. Guizhou Agricultural Sciences, 2022, 50 (7): 21- 25. doi: 10.3969/j.issn.1001-3601.2022.07.004
	丁　洪, 李生秀, 郭庆元, 等. 酸性磷酸酶活性与大豆耐低磷能力的相关研究. 植物营养与肥料学报, 1997, 3 (2): 123- 128. doi: 10.3321/j.issn:1008-505X.1997.02.005
	Ding H, Li S X, Guo Q Y, et al. Studies on the correlation between acid phosphatase activity and low phosphorus tolerance in soybean. Plant Nutrition and Fertilizer Science, 1997, 3 (2): 123- 128. doi: 10.3321/j.issn:1008-505X.1997.02.005
	杜普旋, 刘　浩, 胡冬秀, 等. 花生紫色酸性磷酸酶基因家族的鉴定及表达分析. 中国油料作物学报, 2022, 44 (3): 522- 531.
	Du P X, Liu H, Hu D X, et al. Genome-wide identification and expression analysis of purple acid phosphatase (PAPs) gene family in peanut. Chinese Journal of Oil Crop Sciences, 2022, 44 (3): 522- 531.
	范辉华, 李建民, 陈永滨, 等. 闽楠光合特性测定分析. 西部林业科学, 2016, 45 (5): 49- 53.
	Fan H H, Li J M, Chen Y B, et al. Photosynthetic characteristics of Phoebe bournei. Journal of West China Forestry Science, 2016, 45 (5): 49- 53.
	黄小辉, 夏　鹰, 冯大兰, 等. 缺磷胁迫对核桃幼苗生长及生理特征的影响. 土壤通报, 2022, 53 (3): 613- 622.
	Huang X H, Xia Y, Feng D L, et al. Effects of phosphorus deficiency stress on growth and physiology of walnut seedlings. Chinese Journal of Soil Science, 2022, 53 (3): 613- 622.
	刘　浩, 李玉龙, 汤萌萌, 等. 拟南芥miR156与miR399在低磷胁迫中的对话机制初探. 河南农业科学, 2019, 48 (7): 54- 58.
	Liu H, Li Y L, Tang M M, et al. Initial exploration of cross talk between miR156 and miR399 of Arabidopsis thaliana under low phosphorus stress. Journal of Henan Agricultural Sciences, 2019, 48 (7): 54- 58.
	刘攀道, 黄　睿, 许文茸, 等. 植物紫色酸性磷酸酶的研究进展. 热带作物学报, 2019, 40 (2): 410- 416. doi: 10.3969/j.issn.1000-2561.2019.02.028
	Liu P D, Huang R, Xu W R, et al. Research progress of purple acid phosphatase in plants. Chinese Journal of Tropical Crops, 2019, 40 (2): 410- 416. doi: 10.3969/j.issn.1000-2561.2019.02.028
	庞　欣, 程　远, 郭勤卫, 等. 辣椒紫色酸性磷酸酶基因家族的鉴定与表达特征. 浙江农业学报, 2017, 29 (9): 1498- 1505. doi: 10.3969/j.issn.1004-1524.2017.09.11
	Pang X, Cheng Y, Guo Q W, et al. Genome-wide identification and expression analysis of purple acid phosphatases genes in pepper. Acta Agriculturae Zhejiangensis, 2017, 29 (9): 1498- 1505. doi: 10.3969/j.issn.1004-1524.2017.09.11
	钱　方, 胡利娟, 余　婕, 等. 甘蓝型油菜HIPPs基因家族的鉴定与表达分析. 植物遗传资源学报, 2022, 23 (4): 1- 18.
	Qian F, Hu L J, Yu J, et al. Identification and expression analysis of HIPPs gene family in Brassica napus L. Journal of Plant Genetic Resources, 2022, 23 (4): 1- 18.
	田　江, 梁翠月, 陆　星, 等. 根系分泌物调控植物适应低磷胁迫的机制. 华南农业大学学报, 2019, 40 (5): 175- 185. doi: 10.7671/j.issn.1001-411X.201905068
	Tian J, Liang C Y, Lu X, et al. Mechanism of root exudates regulating plant responses to phosphorus deficiency. Journal of South China Agricultural University, 2019, 40 (5): 175- 185. doi: 10.7671/j.issn.1001-411X.201905068
	田璟鸾. 2013. 水稻紫色酸性磷酸酶Ia亚家族基因的功能研究. 杭州: 浙江大学.
	Tian J L. 2013. Funcitional analysis of genes in rice purple acid phosphatase in subgroup. Hangzhou: Zhejiang University. ［in Chinese］
	魏　铭, 王鑫伟, 陈　博, 等. 植物紫色酸性磷酸酶基因家族功能研究进展. 植物学报, 2019, 54 (1): 93- 101. doi: 10.11983/CBB18044
	Wei M, Wang X W, Chen B, et al. Progress in the functional study of the purple acid phosphatase gene family in plants. Chinese Bulletin of Botany, 2019, 54 (1): 93- 101. doi: 10.11983/CBB18044
	吴梦洁, 洪家都, 李芳燕, 等. 发根农杆菌介导的闽楠遗传转化体系构建与优化. 核农学报, 2023, 37 (8): 1516- 1522. doi: 10.11869/j.issn.1000-8551.2023.08.1516
	Wu M J, Hong J D, Li F Y, et al. Construction and optimization of genetic transformation system mediated by agrobacterium rhizogenes in Phoebe bournei. Journal of Nuclear Agricultural Sciences, 2023, 37 (8): 1516- 1522. doi: 10.11869/j.issn.1000-8551.2023.08.1516
	易　双, 聂　治, 张　啸, 等. 玉米紫色酸性磷酸酶(PAPs)基因家族的鉴定与低磷响应特征. 农业生物技术学报, 2015, 23 (3): 281- 290. doi: 10.3969/j.issn.1674-7968.2015.03.001
	Yi S, Nie Z, Zhang X, et al. Identification of maize (Zea mays) purple acid phosphatase (PAPs) genes family and their characterization responses to phosphorus starvation. Journal of Agricultural Biotechnology, 2015, 23 (3): 281- 290. doi: 10.3969/j.issn.1674-7968.2015.03.001
	阴超燕. 2019. 茶树和杨树中紫色酸性磷酸酶基因的鉴定及对磷铁胁迫的响应. 南京: 南京林业大学.
	Yin C Y. 2019. Identification of purple acid phosphatases (PAPs) genes in tea plants and poplar and their expression responses to phosphorus deficency and excess iron. Nanjing: Nanjing Forestry University. ［in Chinese］
	张俊红, 王　洋, 周生财, 等. 闽楠群体遗传结构分析与核心种质库构建. 林业科学, 2024, 60 (1): 68- 79. doi: 10.11707/j.1001-7488.LYKX20230138
	Zhang J H, Wang Y, Zhou S C, et al. Genetic structure analysis and core germplasm collection construction of Phoebe bournei populations. Scientia Silvae Sinicae, 2024, 60 (1): 68- 79. doi: 10.11707/j.1001-7488.LYKX20230138
	周梦岩, 何冬梅, 李亚超, 等. 紫色酸性磷酸酶在植物响应低磷胁迫中的作用研究进展. 分子植物育种, 2021, 19 (11): 3763- 3770.
	Zhou M Y, He D M, Li Y C, et al. Research progress of the role of purple acid phosphatase in plant response to low phosphorus stress. Molecular Plant Breeding, 2021, 19 (11): 3763- 3770.
	朱亚军. 2017. 闽楠低温胁迫RNA-Seq分析及MYB转录因子家族的鉴定. 杭州: 浙江农林大学.
	Zhu Y J. 2017. RNA-Seq analysis and identification of MYB transcription factor family in Phoebe bournei under low temperature stress. Hangzhou: Zhejiang A & F University. ［in Chinese］
	Bailey T L, Johnson J, Grant C E, et al. The MEME suite. Nucleic Acids Research, 2015, 43, W39- W49. doi: 10.1093/nar/gkv416
	Bhadouria J, Giri J. Purple acid phosphatases: roles in phosphate utilization and new emerging functions. Plant Cell Reports, 2022, 41 (1): 33- 51. doi: 10.1007/s00299-021-02773-7
	Chiou T J, Lin S I. Signaling network in sensing phosphate availability in plants. Annual Review of Plant Biology, 2011, 62, 185- 206. doi: 10.1146/annurev-arplant-042110-103849
	Ding K, Zhang Y T, Yrjälä K, et al. The introduction of Phoebe bournei into Cunninghamia lanceolata monoculture plantations increased microbial network complexity and shifted keystone taxa. Forest Ecology and Management, 2022, 509, 120072. doi: 10.1016/j.foreco.2022.120072
	Ding K, Zhang Y T, Wang L, et al. Forest conversion from pure to mixed Cunninghamia lanceolata plantations enhances soil multifunctionality, stochastic processes, and stability of bacterial networks in subtropical southern China. Plant and Soil, 2023, 488 (1/2): 411- 429.
	Dionisio G, Madsen C K, Holm P B, et al. Cloning and characterization of purple acid phosphatase phytases from wheat, barley, maize, and rice. Plant Physiology, 2011, 156 (3): 1087- 1100. doi: 10.1104/pp.110.164756
	Dissanayaka D M S B, Plaxton W C, Lambers H, et al. 2018. Molecular mechanisms underpinning phosphorus-use efficiency in rice. Plant, Cell & Environment, 41(7): 1483–1496.
	Duff S M G, Sarath G, Plaxton W C. The role of acid phosphatases in plant phosphorus metabolism. Physiologia Plantarum, 1994, 90 (4): 791- 800. doi: 10.1111/j.1399-3054.1994.tb02539.x
	Ham B-K, Chen J Y, Yan Y, et al. Insights into plant phosphate sensing and signaling. Current Opinion in Biotechnology, 2018, 49, 1- 9. doi: 10.1016/j.copbio.2017.07.005
	Han X, Zhang J H, Han S, et al. The chromosome-scale genome of Phoebe bournei reveals contrasting fates of terpene synthase (TPS)-a and TPS-b subfamilies. Plant Communications, 2022, 3 (6): 100410. doi: 10.1016/j.xplc.2022.100410
	Kuang R B, Chan K-H, Yeung E, et al. Molecular and biochemical characterization of AtPAP15, a purple acid phosphatase with phytase cctivity, in Arabidopsis. Plant Physiology, 2009, 151 (1): 199- 209. doi: 10.1104/pp.109.143180
	Li W-Y F, Shao G H, Lam H-M. Ectopic expression of GmPAP3 alleviates oxidative damage caused by salinity and osmotic stresses. New Phytologist, 2008, 178 (1): 80- 91. doi: 10.1111/j.1469-8137.2007.02356.x
	Liang C Y, Wang J X, Zhao J, et al. Control of phosphate homeostasis through gene regulation in crops. Current Opinion in Plant Biology, 2014, 21, 59- 66. doi: 10.1016/j.pbi.2014.06.009
	Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^−ΔΔCT method. Methods, 2001, 25 (4): 402- 408. doi: 10.1006/meth.2001.1262
	Olczak M, Morawiecka B, Watorek W. Plant purple acid phosphatases – genes, structures and biological function. Acta Biochimica Polonica, 2003, 50 (4): 1245- 1256. doi: 10.18388/abp.2003_3648
	McPhie P. The protein protocols handbook. Analytical Biochemistry, 2003, 315 (2): 289.
	Plaxton W C, Tran H T. Metabolic adaptations of phosphate-starved plants. Plant Physiology, 2011, 156 (3): 1006- 1015. doi: 10.1104/pp.111.175281
	Robinson W D, Carson I, Ying S, et al. Eliminating the purple acid phosphatase AtPAP26 in Arabidopsis thaliana delays leaf senescence and impairs phosphorus remobilization. New Phytologist, 2012, 196 (4): 1024- 1029. doi: 10.1111/nph.12006
	Schenk G, Mitić N, Hanson G R, et al. Purple acid phosphatase: a journey into the function and mechanism of a colorful enzyme. Coordination Chemistry Reviews, 2013, 257 (2): 473- 482. doi: 10.1016/j.ccr.2012.03.020
	Tian J L, Wang C, Zhang Q, et al. Overexpression of OsPAP10a, a root-associated acid phosphatase, increased extracellular organic phosphorus utilization in rice. Journal of Integrative Plant Biology, 2012, 54 (9): 631- 639. doi: 10.1111/j.1744-7909.2012.01143.x
	Tran H T, Qian W, Hurley B A, et al. 2010. Biochemical and molecular characterization of AtPAP12 and AtPAP26: the predominant purple acid phosphatase isozymes secreted by phosphate-starved Arabidopsis thaliana. Plant, Cell & Environment, 33(11): 1789–1803.
	Vance C P, Uhde-Stone C, Allan D L. Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. New Phytologist, 2003, 157 (3): 423- 447. doi: 10.1046/j.1469-8137.2003.00695.x
	Vineh M A A, Sabet M S, Karimzadeh G. Effect of AtPAP17 and AtPAP26 genes overexpression on yield and yield components under salt stress in Arabidopsis thaliana plant. Environmental Stresses in Crop Sciences, 2021, 2788, 1725.
	Wang L S, Liu D. Arabidopsis purple acid phosphatase 10 is a component of plant adaptive mechanism to phosphate limitation. Plant Signaling & Behavior, 2012, 7 (3): 306- 310.
	Wang L S, Liu D. Functions and regulation of phosphate starvation-induced secreted acid phosphatases in higher plants. Plant Science, 2018, 271, 108- 116. doi: 10.1016/j.plantsci.2018.03.013
	Wang L S, Lu S L, Zhang Y, et al. Comparative genetic analysis of Arabidopsis purple acid phosphatases AtPAP10, AtPAP12, and AtPAP26 provides new insights into their roles in plant adaptation to phosphate deprivation. Journal of Integrative Plant Biology, 2014, 56 (3): 299- 314. doi: 10.1111/jipb.12184
	Wang L S, Li Z, Qian W Q, et al. The Arabidopsis purple acid phosphatase AtPAP10 is predominantly associated with the root surface and plays an important role in plant tolerance to phosphate limitation. Plant Physiology, 2011, 157 (3): 1283- 1299. doi: 10.1104/pp.111.183723
	Wang X R, Wang Y X, Tian J, et al. Overexpressing AtPAP15 enhances phosphorus efficiency in soybean. Plant Physiology, 2009, 151 (1): 233- 240. doi: 10.1104/pp.109.138891
	Wasaki J, Yamamura T, Shinano T, et al. Secreted acid phosphatase is expressed in cluster roots of lupin in response to phosphorus deficiency. Plant and Soil, 2003, 248 (1/2): 129- 136. doi: 10.1023/A:1022332320384
	Xie Z M, Fong W P, Tsang W K. Engineering and optimization of phosphate-responsive phytase expression in Pichia pastoris yeast for phytate hydrolysis. Enzyme and Microbial Technology, 2020, 137, 109533. doi: 10.1016/j.enzmictec.2020.109533
	Zhang J H, Lin Y, Wu F M, et al. Profiling of microRNAs and their targets in roots and shoots reveals a potential miRNA-mediated interaction network in response to phosphate deficiency in the forestry tree Betula luminifera. Frontiers in Genetics, 2021b, 12, 552454. doi: 10.3389/fgene.2021.552454
	Zhang Q, Wang C, Tian J, et al. 2011. Identification of rice purple acid phosphatases related to posphate starvation signalling. Plant Biology (Stuttgart, Germany), 13(1): 7–15.
	Zhang W Y, Gruszewski H A, Chevone B I, et al. An Arabidopsis purple acid phosphatase with phytase activity increases foliar ascorbate. Plant Physiology, 2008, 146 (2): 431- 440. doi: 10.1104/pp.107.109934
	Zhang Y T, Ding K, Yrjälä K, et al. Introduction of broadleaf species into monospecific Cunninghamia lanceolata plantations changed the soil Acidobacteria subgroups composition and nitrogen-cycling gene abundances. Plant and Soil, 2021a, 467 (1/2): 29- 46.
	Zhou X Y, Zhang X P, Li W K, et al. Electronic effect on bimetallic catalysts: cleavage of phosphodiester mediated by Fe(III)-Zn(II) purple acid phosphatase mimics. Inorganic Chemistry, 2020, 59 (17): 12065- 12074. doi: 10.1021/acs.inorgchem.0c01011
	Zhu S N, Guo Q, Xue Y B, et al. 2024. Impaired glycosylation of GmPAP15a, a root-associated purple acid phosphatase, inhibits extracellular phytate-P utilization in soybean. Plant, Cell and Environment, 47(1): 259–277.

成分Component	加磷 +Pi/(mmol·L^?1)	缺磷 -Pi/(mmol·L^?1)
CaCl₂·2H₂O	0.5	0.5
MgSO₄·7H₂O	0.5	0.5
KCl	0	1.0
KH₂PO₄	1.0	0
H₃BO₃	0.1	0.1
MnCl₂	20×10^?3	20×10^?3
CuSO₄·5H₂O	1×10^?3	1×10^?3
ZnSO₄·7H₂O	3×10^?3	3×10^?3
Na₂MoO₄·2H₂O	1×10^?3	1×10^?3
CoCl₂·6H₂O	1×10^?4	1×10^?4
FeSO₄·7H₂O	0.1	0.1

引物名称Primer	序列Sequence（5’→3’）
PbPAP10a-F	CAGTTGTGATGGGAGTGATGA
PbPAP10a-R	GTGAACCTGTTGTGGAGCAT
PbPAP15a-F	AAAGACCATCCCTACCACCC
PbPAP15a-R	GAGAGGGTGACGGAGATCTG
PbPAP26-F	CTTTTGAGCTCTGTGGGCAA
PbPAP26-R	ACCTTGAGTAATATGGACCTGCT
PbCBP20-F	GAAGGCAGACAATGGG
PbCBP20-R	ATAGTGCGATGGGACAG

引物名称Primer	序列Sequence（5’→3’）
PbPAP10a-F	ATGGGTTCTCCAGTTGTGATG
PbPAP10a-R	TTATGAATACGATTTGGTGGATTCATT
PbPAP15a-F	ATGGATTGGGATTTTCTCTCATTTG
PbPAP15a-R	CTACGCTGGGAAATGCAATT
PbPAP26-F	ATGGTAGAGGCTTATAGCAT
PbPAP26-R	TCAGAATGTGGCAATGCG

蛋白 Protein	基因编号 Gene ID	氨基酸 Amino acid	分子量 MW/kDa	等电点 PI
PbPAP1	Phbou.01G1223.1	613	69.05	6.10
PbPAP2	Phbou.01G1352.1	646	72.98	5.46
PbPAP9	Phbou.01G1351.1	648	73.94	6.16
PbPAP10a	Phbou.01G2906.1	473	54.53	6.55
PbPAP10b	Phbou.12G1471.1	456	52.32	6.70
PbPAP15a	Phbou.01G3123.1	545	62.01	5.51
PbPAP15b	Phbou.08G1918.1	547	61.92	5.26
PbPAP16a	Phbou.01G3288.1	376	42.08	7.13
PbPAP16b	Phbou.01G3289.1	353	39.96	6.00
PbPAP17a	Phbou.01G3733.1	326	36.73	5.43
PbPAP17b	Phbou.02G2250.1	329	37.38	5.25
PbPAP18	Phbou.10G0372.1	437	49.62	5.59
PbPAP20	Phbou.05G2290.1	422	46.90	5.58
PbPAP22	Phbou.04G1339.1	441	49.96	5.89
PbPAP23	Phbou.11G1361.1	543	60.84	5.52
PbPAP26	Phbou.03G0433.1	480	55.30	6.47
PbPAP27a	Phbou.01G3074.1	615	69.27	5.85
PbPAP27b	Phbou.08G1858.1	620	69.73	6.06
PbPAP27c	Phbou.03G0971.1	626	69.75	5.28
PbPAP28	Phbou.03G0037.1	402	45.51	5.80
PbPAP29a	Phbou.08G0566.1	606	66.91	8.49
PbPAP29b	Phbou.07G1718.1	372	41.02	5.36

蛋白 Protein	保守结构域 Domain
PbPAP1	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP2	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP9	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP10a	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP10b	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP15a	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP15b	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP16a		Metallophos
PbPAP16b		Metallophos
PbPAP17a		Metallophos
PbPAP17b		Metallophos
PbPAP18	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP20	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP22	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP23	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP26	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP27a	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP27b	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP27c	Pur_ac_phosph_N	Metallophos	Metallophos_C
PbPAP28		Metallophos
PbPAP29a		Metallophos
PbPAP29b		Metallophos

Analysis of Purple Acid Phosphatases Genes (PAPs) Family in Phoebe bournei and the Functions of Three PAPs under Low Phosphorus Stress

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 16

References 0

Related Articles 12

Recommended Articles

Metrics

Comments

[1]	Huang Jin, Zhang Junhong, Tong Zaikang, Yang Qi. Effects of Artificial Dehydration on Germination Characteristics of Phoebe bournei Seeds [J]. Scientia Silvae Sinicae, 2025, 61(4): 249-256.
[2]	Junhong Zhang,Yang Wang,Shengcai Zhou,Xiaolin Wu,Renchao Wu,Qi Yang,Yuting Zhang,Zaikang Tong. Genetic Structure Analysis and Core Germplasm Collection Construction of Phoebe bournei Populations [J]. Scientia Silvae Sinicae, 2024, 60(1): 68-79.
[3]	Yan Wang,Jinling Feng,Xiaohui Wu,Lanming Huang,Juan Wu,Yu Chen,Zhijian Yang. Effects of Fertilization on Photosynthetic Carbon Fixation of Phoebe bournei Seedlings [J]. Scientia Silvae Sinicae, 2022, 58(5): 40-52.
[4]	Miao Zhang,Shengcai Zhou,Mengjie Wu,Zaikang Tong,Xiao Han,Junhong Zhang,Longjun Cheng. Identification of the PbWRKY Gene Family and Its Expression Analysis under Deficiency of Phosphorus in Phoebe bournei [J]. Scientia Silvae Sinicae, 2022, 58(2): 133-147.
[5]	Xiao Wang,Xiaoli Wei,Gaoyin Wu,Shengqun Chen. Effects of Different Nitrogen Forms and Supply on Photosynthetic Characteristics and Growth of Phoebe bournei Seedlings under Elevated CO₂ Concentration [J]. Scientia Silvae Sinicae, 2021, 57(4): 173-181.
[6]	Zhang Yang, Du Lin, Tang Xianfeng, Liu Huanhuan, Zhou Gongke, Chai Guohua. Identification and Expression Pattern Analyses of Populus BAG Genes [J]. Scientia Silvae Sinicae, 2019, 55(1): 138-145.
[7]	Wang Haibo, Gong Ming, Guo Junyun, Xin Hu, Tang Lizhou, Liu Chao, Gao Yong, Dai Dongqin. Molecular Cloning and Prokaryotic Expression of Orphan Gene Soloist of AP2/ERF Gene Family in Jatropha curcas [J]. Scientia Silvae Sinicae, 2018, 54(9): 60-69.
[8]	Han Shumei, Li Jun, Lü Ruihua, Wang Xiaohong, Liu Changying, Zhao Aichun, Lu Cheng, Yu Maode. Characteristics, Cloning and Expression of the MLX56 Gene Family in Mulberry [J]. Scientia Silvae Sinicae, 2015, 51(4): 60-70.
[9]	Zhao Ying;Zhou Zhichun;Jin Guoqing. GCA/SCA of Seedling Growth and Root Parameters in Pinus massoniana and the Phosphorus Environment Influence [J]. Scientia Silvae Sinicae, 2009, 12(6): 27-33.
[10]	Zhou Zhichun;Xie Yurong;Jin Guoqing;Chen Yue;Song Zhenying. Study on Phosphorus Efficiency of Different Provenances of Pinus massoniana [J]. Scientia Silvae Sinicae, 2005, 41(4): 25-30.
[11]	Xie Yurong;Zhou Zhichun;Liao Guohua;Jin Guoqing;Chen Yue. Difference of Induced Acid Phosphate Activity Under Low Phosphorus Stress of Pinus massoniana Provenances [J]. Scientia Silvae Sinicae, 2005, 41(3): 58-62.
[12]	An Xinmin;Zhang Shanglong;Xu Changjie;Tao Jun. Cloning and Characterization of Members of Acid Invertase Gene Family in Citrus [J]. Scientia Silvae Sinicae, 2004, 40(4): 58-62.