Fig.1

The inhibition rate of V. fordii and V. montana after Fof-1 infection A: The inhibition rate of V. fordii and V. montana after Fof-1 infection from the 2~9 day. B and C: The inhibition effect of roots extract from V. fordii and V. montana. D and E: The ethyl acetate (extractant) control and blank control."

Fig.2

The metabolomics analysis on roots of V. fordii and V. montana after Fof-1 infection A and B: The scores profiles of PCA and PLS-DA analyses on metabolites of root extraction from V. fordii and V. montana, respectively. C: The volcanic plot of 106 up-regulated metabolites and 111 down-regulated metabolites after Fof-1 infection in root of V. montanta. VIP: Variable importance in project. D: The heat map of 106 up-regulated metabolites in V. montana. Red and green bands respectively indicate high and low expression of metabolites in the heat map. The 32 flavonoids were shown in the red boxes. F3-1, F3-2, F3-3 and M3-1, M3-2, M3-3 represent the three independent biological replicates in V. fordii and V. montana."

Table 1

The information of 32 upregulated flavonoids in V. montana root after Fof-1 infection"

Fig.3

The transcriptome analysis between V. fordii and V. montana after Fof-1 infection. A: The up-regulated expression mode of genes in the roots of V. montana upon the Fof-1 infection at the early stage (F1, M1). M0 and F0 represent uninfected V. montana and V. fordii; M1 and F1 represent the early stage of Fof-1 infection; M2 and F2 represent the middle stage of infection; M3 and F3 represent the late stage of infection. B: The scatter diagram of KEGG enrichment on differentially expressed genes at the early stage of Fof-1 infection."

Table 2

The information of differentially expressed genes involved in the pathways of phenylpropanoid and flavonoids biosynthesis in the roots of V. montana"

Fig.4

Gene network analysis of hub genes in phenylpropanoid biosynthesis pathways responding to Fof-1 infection in V. montana. A: Gene network of four hub genes correlated with other 1 625 genes in phenylpropanoid biosynthesis pathways responding to Fof-1 infection in V. montana. The red V represents the hub genes and the black spots represent the correlated 1 625 genes. B: The KEGG annotation for the 1 625 genes."

Fig.5

Expression mode identification of 4 hub genes by qRT-PCR in V. montana. M0 represents uninfected V. montana; M1 represents the early stage of Fof-1 infection; M2 represents the middle stage of infection; M3 represents the late stage of infection. The heatmap up the histogram shows the relative gene expression level by RNA seq. Red represents the higher expression level; Green represents the lower expression level."