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Scientia Silvae Sinicae ›› 2022, Vol. 58 ›› Issue (2): 159-170.doi: 10.11707/j.1001-7488.20220216

• Research papers • Previous Articles     Next Articles

Response of Flavonoids Biosynthesis in Roots of Vernicia montana to Fusarium Wilt Infection

Jia Wang1,2,Xiaojie Liang1,Ming Gao1,Liwen Wu1,Yunxiao Zhao1,Yangdong Wang1,Shiqing Huang3,Yongzhi Zhang3,Huoyong Fu3,Yicun Chen1,*   

  1. 1. Research Institute of Subtropical Forestry, CAF Hangzhou 311400
    2. Nanjing Forestry University Nanjing 210037
    3. Anji Longshan Forest Farm Huzhou 313306
  • Received:2020-12-21 Online:2022-02-25 Published:2022-04-26
  • Contact: Yicun Chen


Objective: Objective: The tung oil tree, Vernicia fordii, is suffered from the soil-borne Fusarium oxysporum f. sp. fordii, Fof-1. However its sister species, Vernicia montana, shows high resistance to the pathogen. This study aims to investigate the resistance mechanism of V.montana, so as to provide ideas for resistance breeding of V. fordii. Method: The ethyl acetate extraction method was used to extract the root metabolites of V.montana and V. fordii after infection. The ultra performance liquid chromatography and tandem mass spectrometry were used to detect the root metabolites. The Illumina HiSeqTM2000 was used to detect the changes of gene expression mode and the pathway in the roots of V.montana and V. fordii during the infection by the Fusarium wilt pathogen. The gene expression was further validated by qRT-PCR method. The data were analyzed using R soft package and other bioinformatics methods. Result: 1) Comparing with that of V. fordii, the root extract of V. Montana had an obvious inhibition effect on the growth of Fof-1. 2) After being infected, the isoflavone and flavanone, such as formononetin 7-O-glucoside (Ononin) and hesperetin 7-rutinoside (hesperidin), in the roots of V. montana were 1 000 folds higher than those in the roots of V. fordii. 3) The phenylpropanoid biosynthesis pathway, the upstream key pathway responsible for flavonoid biosynthesis, was significantly enriched in the roots of resistant V. montana upon Fof-1 infection. 4) There were four hub genes, including 4-coumarate: CoA ligase (4CL), β-D-xylosidase, β-glucosidase, and peroxidase N1 with high connection with the other 1625 genes, which were all up-expressed in the early stage of Fof-1 infection in the phenylpropanoid biosynthesis pathway. Conclusion: Based on the metabonomic and transcriptomic analyses, it is revealed that the phenylpropanoid biosynthesis pathway actively responds to Fof-1 infection in the roots of V. montna, and the oflavone and flavanone, such as on onin and hesperidin, are correspondingly produced for the resistance to Fusarium pathogen Fof-1 in the roots of V. montana.

Key words: tung oil trees, Fusarium wilt disease, flavonoids, phenylpropanoid biosynthesis, hub genes

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