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Scientia Silvae Sinicae ›› 2013, Vol. 49 ›› Issue (10): 127-134.doi: 10.11707/j.1001-7488.20131020

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Comparison of Flavonoids in the Leaves of Three Genera of Bamboo

Wei Qi, Yue Yongde, Tang Feng, Sun Jia   

  1. International Center for Bamboo and Rattan Beijing 100102
  • Received:2012-07-13 Revised:2013-05-06 Online:2013-10-25 Published:2013-11-05

Abstract:

Isoorientin, orientin, isovitexin, vitexin and tricin in the leaves of three genera 11 bamboos been compared by a simple high-performance thin-layer chromatographic (HPTLC) method. The multistage development was performed in Automated Multiple Development (AMD2) and solvents with different ratios were used as mobile phase. The developed plates were scanned by TLC Scanner3. The components were separated well and RF of the five flavonoids 0.25, 0.32, 0.38, 0.47 and 0.88 respectively. The method was validated for precision, accuracy, selectivity and repeatability, and expressed as coefficient of variation (CV[%]). The kinds of flavonoids and the concentrations in the eleven species bamboo of three different genera were significantly different. Phyllostachys nigra the maximum total flavonoids of 0.132%, and the minimum of 0.015%. The results would be useful in extracting natural bamboo-leaf flavonoids.

Key words: HPTLC, bamboo-leaf flavonoids, qualitative and quantitative analysis, Bambusa, Dendrocalamus

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