团花树α扩展蛋白基因的克隆及表达分析

Abstract

Abstract:

Fifteen cDNAs, designated as AcEXPA 2 to AcEXPA16 (GenBank accession No. JF922686-JF922700) with the corresponding genomic DNA sequences (GenBank accession No. JF922701-JF922715), were isolated by amplifying conserved domain binding with genomic walking and 3'RACE techniques from four differential tissues in Anthocephalus chinensis. The size and sequence of these α-expansin homologues including known AcEXPA1 were highly conserved, and had the same sequence structures as an N-terminal signal peptide, three exons and two introns. Their amino acid alignment and phylogenetic analysis showed that A. chinensis α-expansin genes were divided into four subgroups: A, B, C and D. A real-time quantitative PCR analysis showed that the transcript level of AcEXPA 1-16 was redundant in the same tissue and tissue-specific with each gene in different tissue, especially, the transcript level of AcEXPA 8 extremely high in the cambium region. These results suggest that AcEXPA 8 may play an important role in xylogenesis. This study on α-expansin genes from A. chinensis will lay the foundation for studying the relationship between α-expansin genes and growth rate and wood quality of the xylem in this fast-growing tree, and finally provide potential candidate genes for tree molecular breeding.

Key words: Anthocephalus chinensis, α-expansin, gene cloning, RT-qPCR

CLC Number:

S718.46

Ouyang Kunxi, Li Juncheng, Huang Hao, Liu Mingqian, Chen Xiaoyang. Molecular Cloning and Expression Analysis of α-Expansin Genes in Anthocephalus chinensis[J]. Scientia Silvae Sinicae, 2013, 49(9): 62-71.

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Molecular Cloning and Expression Analysis of α-Expansin Genes in Anthocephalus chinensis

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