光皮桦BlSPL1转录因子基因的克隆、表达及单核苷酸多态性分析

Abstract

Abstract:

Squamosa promoter binding protein like genes(SPLs)play an important role in regulating flowering of plants. In this study, one SPL gene named BlSPL 1 was cloned from Betula luminifera using RACE (rapid-amplification of cDNA ends) strategy. The cDNA of BlSPL 1 is 1 825 bp in length with an open reading frame (ORF) encoding a protein of 389 aa. The deduced protein sequence of the BlSPL 1 shares the highest identity with PpSPL 1 (EMJ10405) (67%) and belongs to group Ⅶ of SPL family from land plants. Expression analysis indicated that the BlSPL 1 transcripts were at a relatively low level in male flower and seedlings, but were apparently up-regulated during the development from buds to female flowers, which implied that BlSPL 1 might play important roles in early stage of female flower formation. At the same time, the corresponding genomic fragments of BlSPL 1 were also cloned from 57 different germplasms for the single nucleotide polymorphism (SNP) analysis. A total of 98 SNPs were detected, and the frequency and diversity of SNPs were 1/26 bp and 0.007 12, respectively. There were 28 SNPs detected in the exons of BlSPL 1, among which, there were 11 synonymous and 17 missense mutations SNPs, respectively. Linkage disequilibrium (LD) analysis showed that LD declined rapidly within the BlSPL 1 along with the length increasing in different population of B. luminifera, suggesting that LD mapping based on BlSPL 1 gene would be feasible and useful in B. luminifera.

Key words: Betula luminifera, transcription factor, SPL genes, single nucleotide polymorphism

CLC Number:

S718.46

Li Yuling, Zhou Houjun, Lin Erpei, Huang Huahong, Xu Lili, Tong Zaikang. Isolation, Expression and Single Nucleotide Polymorphisms Analysis of Transcription Factor (BlSPL 1) from Betula luminifera[J]. Scientia Silvae Sinicae, 2013, 49(9): 52-61.

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Isolation, Expression and Single Nucleotide Polymorphisms Analysis of Transcription Factor (BlSPL 1) from Betula luminifera

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