香樟未成熟合子胚体胚发生及植株再生研究

doi:10.11707/j.1001-7488.20060607

Scientia Silvae Sinicae ›› 2006, Vol. 42 ›› Issue (6): 37-39.doi: 10.11707/j.1001-7488.20060607

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Study on Somatic Embryogenesis and Plant Regeneration of Immature Zygotic Embryo of Cinnamomum camphora

Du Li^1,2,Ye Yaomei¹,Bao Manzhu¹

1.Key Lab of Horticultural Plant Biology of Ministry of Education College of Horticulture and Forestry Sciences, Huazhong Agricultural University Wuhan 430070;2.China-UK Nanyang Normal University_Rothamsted Research Joint Lab of Insect Biology Nanyang Normal University Nanyang 473000

Received:2004-11-19 Revised:1900-01-01 Online:2006-06-25 Published:2006-06-25

Abstract

Abstract:

Somatic embryogenesis was successfully induced from immature embryos of Cinnamomum camphora. The best carbohydrate form was sucrose under the condition of MS+1.0 mg·L^-1 BA + 0.1 mg·L^-1 2,4-D + 700 mg·L^-1 CH. After subculture of 2 years, embryogenic calli were observed on the somatic embryos. Embryogenic calli were transferred onto induction medium for somatic embryos initiation, maturation medium for somatic embryo maturing, and then to germination medium for shoot induction. The embryogenic calli could be induced to develop into plantlet and successfully transferred to outdoor environment.

Key words: Cinnamomum camphora, immature zygotic embryo, somatic embryo, embryogenic calli, plant regeneration

Du Li;Ye Yaomei;Bao Manzhu. Study on Somatic Embryogenesis and Plant Regeneration of Immature Zygotic Embryo of Cinnamomum camphora[J]. Scientia Silvae Sinicae, 2006, 42(6): 37-39.

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Study on Somatic Embryogenesis and Plant Regeneration of Immature Zygotic Embryo of Cinnamomum camphora

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