ACC合酶cDNA克隆及其对美洲黑杨体内乙烯产生的反义抑制

Abstract

Abstract:

A 1.5 kb fragment of ACC synthase cDNA fragment prepared from total RNA of soybean was amplified by polymerase chain reaction (PCR) and cloned into pGEM^®-T vector. The cloned ACC synthase gene was further inserted into a binary vector, pBin438, in an inverted orientation between the CaMV 35S promoter and Nos 3'termination sequence (pBACS). Transgenic poplar plants were obtained by regeneration of Agrobacterium-mediated transformation of leaves of Populus deltoides. PCR and Southern blotting analyses confirmed the integration of a single antisense ACC synthase gene in transformed poplar genome. The results form reverse transcription PCR (RT-PCR) of RNAs isolated from transgenic poplar leaves confimed that the antisense RNA of ACC synthase presented in these transgenic plants. The amount of ethylene released from transgenic poplar was reduced significantly to about 22% of that released from non-transformed control poplars.

Key words: ACC(l-aminocyclopropane-l-carboxylate)synthase, Gene, Antisense RNA, Populus deltoides

Mingliang Li,Yifan Han,Deyou Qiu,Ning Li,Yingchuan Tian. CLONING OF ACC SYNTHASE cDNA AND ITS INHIBITION OF ETHYLENE SYNTHESIS BY ITS ANTISENSE RNA IN TRANSGENIC POPULUS DELTOIDES[J]. Scientia Silvae Sinicae, 1999, 35(3): 10-15.

Figures/Tables 5

Fig.1

Fig.2

Fig.3

Fig.4

Fig.5

References 0

	陈颖, 韩一凡, 李玲, 等. 苏云金杆菌杀虫晶体蛋白基因转化美洲黑杨的研究. 林业科学, 1995. 31 (2): 97- 103.
	刘传银, 田颖川, 沈全光, 等. 番茄ACC合成酶cDNA克隆及其对果实成熟的反义抑制. 生物工程学报, 1998. 14 (2): 139- 146.
	Adams D O , Yang S F . Ethylene biosynthesis: identification of l-aminocyclo-propane-l-carboxylic acid as an intermediate in the conversion of methionine to ethylene. Proc Natl Acad Sci, USA, 1979. 76 (1): 170- 174. doi: 10.1073/pnas.76.1.170
	Brady C, McGlsson B, Speirss J. Tomato Biotecechnology. Ed Nevins D J, Richard A J. NY: INFO, 1987
	Boller T , Herner R C , Kende H . Assay for and enzymatic formation of an ethylene precursor, l-aminocyclopropane-l-carboxylic acid. Planta, 1979. 145 (3): 293- 303. doi: 10.1007/BF00454455
	Barry C S , Blume B , Bouzayen M , et al. Differential expresion of the l-aminocyclopropane-l-carboxylate oxidase gene family of tomato. Plant J, 1996. 9, 525- 535. doi: 10.1046/j.1365-313X.1996.09040525.x
	Dayton H W , Richard B M , Scott A M . Expression of foreign genes in transgenic yellow-poplar plants. Plant Physiol, 1992. 98 (1): 114- 120. doi: 10.1104/pp.98.1.114
	Inaba A , Nakamura R . Effect of exogenous ethylene concentration and fruit temperature on the minimum treatment time necessary to induce ripening in banana fruit. J Japan, Soc Hort Sci, 1986. 55, 348- 354. doi: 10.2503/jjshs.55.348
	Jehan H , Brown S , Marie D , et al. Ontogenesis and ploidy level of plantlets regenerated from Populus trichocarpa×P. deltoides cv. Hunnegem root, leaf and stem explants. J Plant Physiol, 1994. 114, 576- 585.
	John I , Drake R , Farrell A , et al. Delayed leaf senescence in ethylene-deficient ACC-oxidase antisense tomato plants: Molecular and physiological analysis. Plant Journal, 1995. 7 (3): 483- 490. doi: 10.1046/j.1365-313X.1995.7030483.x
	Lincoln J E , Cordes S , Read E , et al. Regulation of gene expression by ethylene during Lycopersicon esculetm (tomato) fruit development. Proc Natl Acad Sci, USA, 1987. 84 (8): 2793- 2797.
	Liu Y , Hoffman N E , Yang S F . Production by ethylene of the capability to convert l-aminocyclopropane-l-carboxylic acid to ethylene in precli-macteric tomato and cantaloupe fruits. Plant Physiol, 1985. 77 (2): 407- 411. doi: 10.1104/pp.77.2.407
	Liu D , Li N , Dube S , et al. Molecular characterization of a rapidly and transien tly wound-induced soybean gene encoding l-aminocyclop ropane-l-caeboxylate synthase. Plant Cell Physiol, 1993. 34 (7): 1151- 1157.
	Oeller P W , Taylor L P . Reversible inhibition of tomato fruit senescence by antisense RNA. Science, 1991. 254, 437- 439. doi: 10.1126/science.1925603
	Rogers S O , Bendich A J . Extraction of DNA from miligram amounts of fresh, herbarium and mummified plant tissues.Plant Mol. Belgium: Biol. Kluw er Academic Publishers. 1988. A6, 1- 10.
	Sambrook J . Molecular Cloning-A Labotary Manual. (2nd edition) NY: Cold Spring Harbor Laboratory Press.
	van Wounter G D , Anthony D . Abscission of flowers and floral parts. J Exp Bot, 1997. 48, 821- 837. doi: 10.1093/jxb/48.4.821
	Yang S F , Hoffman N E . Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol, 1984. 35, 155- 189. doi: 10.1146/annurev.pp.35.060184.001103
	Yu Y , Adams A D , Yang S F . l-aminocyclopropane carboxylate syn thase, a key enzyme in ethylene biosynthesis. Arch. Biochem. Biophys, 1979. 198 (1): 280- 286. doi: 10.1016/0003-9861(79)90420-X

[1]	Cun Chen,Changjun Ding,Jing Zhang,Bo Li,Yanguang Chu,Xiaohua Su,Qinjun Huang. Population Structure Analysis and Core Collection Construction of Populus deltoides [J]. Scientia Silvae Sinicae, 2020, 56(9): 67-76.
[2]	Yunpeng Wang,Rui Zhang,Zhichun Zhou,Shaohua Huang,Lizhen Ma,Huihua Fan. Dynamic Patterns of Genetic Variation in Early Growth Traits of the Open-Pollinated Families of Schima superba Plus Tree [J]. Scientia Silvae Sinicae, 2020, 56(9): 77-86.
[3]	Wenjuan Wu,Chunyang Zou,Lijing Huang,Yongcan Jin. Dissolution and Regeneration of Bamboo in LiCl/DMSO Solvent System [J]. Scientia Silvae Sinicae, 2020, 56(9): 201-206.
[4]	Lei Wang,Yifan Liang,Junqian Yang,Bingbing Zhang,Tao Wang,Xiuzhen Shi,Hangwei Hu,Zhiqun Huang. Characteristics of Soil nitrogen Retention and Related Functional Microorganism in Soils of Main Afforestation Species in Subtropical Region [J]. Scientia Silvae Sinicae, 2020, 56(8): 27-37.
[5]	Huanhuan Chen,Gexi Xu,Fanqiang Ma,Shun Liu,Miaomiao Zhang,Xiangwen Cao,Jian Chen,Guangdong Zhao,Hongguo Yang,Zuomin Shi. Phylogenetic Structure of Undergrowth Layers across Subalpine Dark Coniferous Forests and Their Post-Harvesting Secondary Forests in Western Sichuan [J]. Scientia Silvae Sinicae, 2020, 56(7): 1-11.
[6]	Weibo Sun,Xindong Gong,Yan Zhou,Hongyan Li. Photosynthetic Characteristics of Transgenic Poplars with Maize PEPC and PPDK Gene at Young Plant Stage [J]. Scientia Silvae Sinicae, 2020, 56(7): 33-43.
[7]	Yihong Meng,Gangbiao Xu,Mengzhu Lu,Xiaolong Jiang,Feilong Guo. Population Genetic Structure and Demographic History of Disanthus cercidifolius var. longipes [J]. Scientia Silvae Sinicae, 2020, 56(7): 55-62.
[8]	Han Peng,Shouke Zhang,Xiansheng Geng,Linxin Fang,Wei Zhang,Jinping Shu,Haojie Wang. Population Differentation of Batocera lineolata(Coleoptera: Cerambycidae) Adults Whose Larvae Fed on Different Host Tree Species [J]. Scientia Silvae Sinicae, 2020, 56(7): 91-103.
[9]	Xingmei Zhang,Yuhan Qi,Ding Ren,Guofu Qu,Yucheng Zhou. Synchronization Control of the Hot Platen Lifting System in Continuous Flat Press Based on Predictive Control [J]. Scientia Silvae Sinicae, 2020, 56(6): 83-93.
[10]	Liming Bian,Huichun Zhang. Application of Phenotyping Techniques in Forest Tree Breeding and Precision Forestry [J]. Scientia Silvae Sinicae, 2020, 56(6): 113-126.
[11]	Zong Zhao,Yong Liu,Zuwei Tian,Hongyan Jia,Ruirui Zhao,Ning An. Dynamics of Seed Rain, Soil Seed Bank and Seedling Regeneration of Castanopsis hystrix [J]. Scientia Silvae Sinicae, 2020, 56(5): 37-49.
[12]	Ming Jiang,Shisheng Ke,Junfeng Wang. Characterization and Phylogenetic Analysis of Ostrya multinervis Chloroplast Genome [J]. Scientia Silvae Sinicae, 2020, 56(5): 60-68.
[13]	Zhongyuan Liu,Zheng Liu,Ying Xu,Shanshan Liu,Zhilan Tian,Qingjun Xie,Caiqiu Gao. Cloning and Salt Tolerance Analysis of Transcription Factor HSFA4 from Betula platyphylla [J]. Scientia Silvae Sinicae, 2020, 56(5): 69-79.
[14]	Yuchen Yan,Zejian Li,Guihong Li,Meicai Wei. A New Species of Zaraea(Leach, 1817)(Hymenoptera, Cimbicidae) from China Harming Padus brachypoda [J]. Scientia Silvae Sinicae, 2020, 56(5): 105-112.
[15]	Sifan Shen,Zhen Zhang,Xiangbo Kong,Fu Liu,Sufang Zhang. Research Techniques of Insect Odour Receptors and Their Application in Forest Insects [J]. Scientia Silvae Sinicae, 2020, 56(5): 150-159.

CLONING OF ACC SYNTHASE cDNA AND ITS INHIBITION OF ETHYLENE SYNTHESIS BY ITS ANTISENSE RNA IN TRANSGENIC POPULUS DELTOIDES

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 0

Related Articles 15

Recommended Articles

Metrics

Comments