长柄双花木种群遗传结构及种群历史

doi:10.11707/j.1001-7488.20200706

Abstract

Abstract:

Objective: Population genetic diversity and genetic structure of Disanthus cercidifolius var. longipes were analyzed,and its population demographic history was speculated,so as to deepen understanding on its evolutionary process,and to provide a theoretical basis for conservation and utilization of its genetic resources. Method: Genetic diversity of 261 individuals from 12 natural populations of the species were estimated at 15 polymorphic loci using TP-M13-SSR technology. All sampled individuals were genotyped using Gene Marker,genetic parameters were assessed with FSTAT,analysis of molecular variance was carried out with ARLEQUIN,Bayesian clustering analysis was conducted for all sampled individuals with STRUCTURE,and genetic bottleneck effect was detected by Bottleneck software. Result: A total of 129 alleles were detected at 15 loci,and the mean allele number and effective allele number at each locus were 8.6 and 3.5,respectively. The mean observed heterozygosity and expected heterozygosity were 0.37,0.67. At population level,the mean allele number and effective allele number were 3.4 and 2.1,the mean observed heterozygosity and expected heterozygosity were 0.35 and 0.43,respectively. The private allelic richness and within-population inbreeding coefficient were 0.15 and 0.19,respectively. All populations deviated significantly from HWE with an excess of homozygotes except DX population. The genetic differentiation between populations was statistically significant(P < 0.001). All the populations were classified into two clusters,and the lineages of most individual were clear. The populations had not experienced genetic bottleneck events over the historical evolutionary process. Conclusion: Our results demonstrated that D. cercidifolius var. longipes maintained a moderate to high genetic variation,and the habitat fragmentation and genetic drift has not yet declined within-population genetic diversity. This species has high evolutionary potential. The geographic isolation,habitat fragmentation caused by climate change and human disturbance might be the main causes for the formation of current geographical distribution pattern and population genetic structure. Overall,our study provides scientific evidences for making effective conservation strategies for the species.

Key words: Disanthus cercidifolius var. longipes, fluorescent SSR, population genetic structure, population demographic history

CLC Number:

S718.46

Yihong Meng,Gangbiao Xu,Mengzhu Lu,Xiaolong Jiang,Feilong Guo. Population Genetic Structure and Demographic History of Disanthus cercidifolius var. longipes[J]. Scientia Silvae Sinicae, 2020, 56(7): 55-62.

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种群Populations	纬度 Latitude(N)	经度 Longitude(E)	海拔 Altitude/m	样本量 Sample size	N_A	N_E	H_O	H_E	p_AR	F_IS
道县Daoxian(DX)	25°33′	111°20′	1 190~1 200	20	3.5	2.3	0.43	0.44	0.18	-0.02
莽山Mangshan(MS)	24°58′	112°53′	940~945	25	2.7	1.7	0.28	0.37	0.06	0.25^**
宜章Yizhang(YZ)	25°25′	112°49′	890~895	23	2.8	1.9	0.35	0.38	0.07	0.12^**
连州Lianzhou(LZ)	24°55′	112°40′	1 015~1 025	21	2.9	2.0	0.40	0.44	0.14	0.10^**
新宁Xinning(XN)	26°25′	110°36′	260	25	3.2	2.0	0.36	0.44	0.20	0.15^**
邵阳Shaoyang(SY)	26°43′	111°18′	735~745	15	3.3	1.9	0.24	0.37	0.18	0.40^**
宜黄Yihuang(YH)	27°14′	116°21′	870~880	24	3.3	2.2	0.30	0.42	0.10	0.19^**
宜丰Yifeng(YF)	28°33′	114°35′	595~659	28	3.6	2.0	0.29	0.43	0.18	0.39^**
龙泉Longquan(LQ)	28°06′	118°51′	1 070~1 080	20	3.1	2.2	0.36	0.45	0.13	0.16^**
开化Kaihua(KH)	29°23′	118°13′	480~485	22	3.3	2.1	0.39	0.44	0.11	0.21^**
玉山Yushan(YS)	28°54′	118°03′	1 240~1 310	25	3.8	2.4	0.38	0.50	0.25	0.23^**
井冈山Jinggangshan(JGS)	26°47′	113°58′	300~310	26	4.1	2.5	0.47	0.52	0.22	0.15^**
平均Mean					3.4	2.1	0.35	0.43	0.15	0.19

位点Locus	引物序列Primer sequence(5′-3′)		N_A	N_E	H_O	H_E	HWE
DC013	F: TCCATCAAGCCAGAATCAGA	R: TGAATTTGGTGGTCGCAATA	12	4.9	0.62	0.80	D
DC102	F: AAACACTTGGCGTGATTTCC	R: ACCGGGAAGGAGAAAAAGAA	6	2.0	0.25	0.50	D
DC101	F: CATCTGGCAGTTGGACTTGA	R: CGCTCCAATACTGCAAGTGA	13	6.8	0.56	0.85	D
DC70	F: GACACAGAGAGGCAATCGG	R: AGCAGAAGCTTGTAGGGTGG	7	2.7	0.35	0.63	D
DC67	F: ATATTGCTGTTCAGGCCGAC	R: AGTTGCCCTTTAGCTCTCCA	8	4.5	0.44	0.78	D
DC111	F: TTCGAGAGTTTGAAATGGGG	R: CCAAGCACTTCAATGCCTTT	4	2.3	0.20	0.56	D
DC93	F: ATCCCCCACCAAATCAAAAT	R: ATCAAAACCGCAAATGGAAC	9	2.1	0.40	0.51	D
DC95	F: GCATAAGCTGGACCCATTTC	R: GCATTTCCTTTTTGTGATGCT	7	3.1	0.21	0.68	D
DC115	F: TGCCGATCATGAACAAAAAG	R: TTTCTCAGCAACCAAACGGT	15	4.6	0.46	0.79	D
DC408	F: CCGTGAATTTTTCCACCAAT	R: CCAACCGAATCTCACTCGTT	5	3.4	0.55	0.71	D
DC82	F: AGCAGTTTTTGCCTCTTGGA	R: AAAAAGGGGAATCAACCCAG	6	2.6	0.31	0.62	D
DC55	F: GAATGAGGGTCTCCGGATTT	R: CGAAAACGAACGATGACTGA	10	2.5	0.27	0.60	D
DC71	F: TCTCTTGGTTTGGTGAAGGG	R: GCTGAAAACAGTGCAGGTGA	11	4.8	0.36	0.79	D
DC57	F: GCTACAACATTTTCAGCGCA	R: CTCTCCTTCCCCAAGCTTCT	5	2.0	0.11	0.51	D
DC87	F: GGGACAGAGGTGTAAATGCG	R: GGACAGAGAGAGATGAGGCG	11	3.7	0.39	0.73	D
平均Mean			8.6	3.5	0.37	0.67

种群Populations	DX	MS	YZ	LZ	XN	SY	YH	YF	LQ	KH	YS
MS	0.354
YZ	0.351	0.414
LZ	0.257	0.207	0.397
XN	0.304	0.400	0.465	0.274
SY	0.378	0.348	0.291	0.400	0.492
YH	0.352	0.372	0.496	0.296	0.252	0.428
YF	0.407	0.406	0.392	0.300	0.314	0.351	0.324
LQ	0.376	0.514	0.408	0.439	0.431	0.427	0.384	0.347
KH	0.405	0.467	0.344	0.398	0.425	0.371	0.451	0.395	0.250
YS	0.350	0.440	0.331	0.349	0.409	0.356	0.397	0.369	0.245	0.169
JGS	0.357	0.379	0.362	0.307	0.342	0.341	0.304	0.296	0.278	0.242	0.230

变异来源 Source of variation	df	均方和 Sum of squares	方差组分 Variance component	变异百分比 Percentage variation(%)	P
种群间Among populations	11	364.904	0.738 9	40.33	＜0.001
种群内Within population	510	557.437	1.093 0	59.67
整体Total	521	922.342

种群 Population	N_LHE	P	漂移模型测试 Mode-shift test
DX	6.42	0.289	L-shaped
MS	7.35	0.163	L-shaped
YZ	6.98	0.318	L-shaped
LZ	7.66	0.134	L-shaped
XN	7.96	0.948	L-shaped
SY	7.07	0.966	L-shaped
YH	6.87	0.259	L-shaped
YF	7.43	0.863	L-shaped
LQ	7.19	0.055	L-shaped
KH	7.86	0.476	L-shaped
YS	8.06	0.138	L-shaped
JGS	8.55	0.360	L-shaped

Population Genetic Structure and Demographic History of Disanthus cercidifolius var. longipes

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