氮添加与干旱共同作用下的油松幼苗渗透调节机制

doi:10.11707/j.1001-7488.LYKX20240362

Abstract

Abstract:

Objective: This study explored the effect of nitrogen addition on the content of osmoregulatory substances in a typical tree species, Pinus tabuliformis, in the Loess Plateau region, for revealing the mechanism by which nitrogen addition alleviates drought stress by maintaining hydraulic function, and thereby exploring the response of forest ecosystems in the region to global change. This research aimed to provide a scientific basis for managing P. tabuliformis plantations in the Loess Plateau region. Method: Two-year-old P. tabuliformis seedlings were used as the research object, and four levels of drought stress treatments (control CK, mild stress RE₂₅, moderate stress RE₅₀, and severe stress RE₇₅) and three nitrogen addition levels (N₀, N₃, N₆ with concentrations of 0, 3, 6 g·m^–2a^–1, respectively) were set up to analyze the changes in physiological growth characteristics, osmoregulatory substances, and hydraulic function of the seedlings under the interactive effects of nitrogen addition and drought stress, along with their interrelationships. Result: 1） Under CK treatment, the total biomass at N₃ and N₆ levels significantly increased by 3.69% and 10.56% (P<0.05) compared to the N0 level, and the net photosynthetic rate increased by 22.16% and 79.61% (P<0.05). At the N₀ level, the root-to-shoot ratio under RE₇₅ treatment significantly increased by 54.9% (P<0.05) compared to the CK group. 2） Correlation analysis proved that high-concentration nitrogen application significantly increased the proline content in new branches under CK treatment, mild and moderate drought stress; nitrogen application significantly increased the content of non-structural carbohydrates under each drought treatment but had no significant effect on the content of potassium ions. The effect of nitrogen application on osmotic regulation of new branches of P. tabuliformis seedlings was greater than that of drought. 3） Under nitrogen application conditions, the total amount of soluble sugars and non-structural carbohydrates showed a significant positive correlation with net photosynthetic rate, biomass, hydraulic conductivity, and water use efficiency, indicating that nitrogen application increased the formation of soluble substances and non-structural carbohydrates by enhancing the net photosynthetic rate above ground, promoting biomass accumulation, and maintaining hydraulic function through osmotic regulation by soluble sugars, thereby improving the water use efficiency of P. tabuliformis. 4） Redundancy analysis showed that proline (P<0.05) and soluble sugar (P<0.05) had significant effects on hydraulic function under the coupled effects of nitrogen addition and drought stress, with an explanation rate of 38.5% and 12.9%, respectively. Conclusion: New branches are the most vulnerable organs under drought stress. Among nitrogen addition levels, the concentration of 6 g·m^?2a^?1 is the optimal nitrogen addition level for alleviating mild drought stress in P. tabuliformis seedlings.

Key words: drought stress, nitrogen addition, hydraulic conductivity, Pinus tabuliformis, osmotic regulation

CLC Number:

Yan Zeng,Jirong Mao,Xianglin Chen,Xinyu Xu,Jing Liang,Ying Liu. Osmotic Regulation Mechanism of Pinus tabuliformis Seedlings under the Joint Effects of Nitrogen Addition and Drought Stress[J]. Scientia Silvae Sinicae, 2025, 61(8): 70-79.

Figures/Tables 7

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References 0

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指标 Indicator	干旱胁迫处理 Drought stress treatments	氮添加水平 Nitrogen addition levels
指标 Indicator	干旱胁迫处理 Drought stress treatments	N₀	N₃	N₆
净光合速率 Net photosynthetic rate/(μmol?m^–2s^–1)	CK	5.64±0.13Bb	6.56±0.75Bab	10.22±0.04Aa
	RE₂₅	8.11±0.50Aab	10.05±0.07Ab	10.27±0.04Aa
	RE₅₀	5.33±0.01Bb	5.56±0.01BCb	6.77±1.06Ba
	RE₇₅	4.94±0.05Ba	5.30±0.04Ca	5.45±0.53Ba
水分利用效率 Water use efficiency/(μmol?mol^–1)	CK	8.63±0.22Bb	11.75±1.68Bb	16.28±0.07Aa
	RE₂₅	11.03±0.67Ab	15.99±0.13Aab	16.36±0.06Aa
	RE₅₀	10.88±0.01Aa	10.86±0.01Ba	11.63±1.41Ba
	RE₇₅	7.46±0.09Ca	8.06±0.06Ca	7.64±0.62Ca
叶面积 Leaf area/m²	CK	0.65±0.02Cc	0.81±0.01Cb	1.14±0.04Ca
	RE₂₅	0.75±0.03Bc	0.95±0.05Bb	1.52±0.04Aa
	RE₅₀	1.04±0.04Ab	1.09±0.03Ab	1.32±0.04Ba
	RE₇₅	0.57±0.03Cc	0.92±0.04BCb	1.21±0.03BCa
总生物量 Total biomass/g	CK	118.71±1.61Bb	123.09±3.47Ba	131.05±2.33Ba
	RE₂₅	116.82±2.88Bc	129.83±6.76Ab	156.41±2.07Aa
	RE₅₀	125.15±5.93Ab	126.01±6.99Ab	155.35±4.26Aa
	RE₇₅	90. 67±3.55Cc	115.95±1.48Bb	128.49±2.54Ca
根冠比 Root-shoot ratio/(g?g^–1)	CK	0.153±0.009Cb	0.167±0.013Ca	0.177±0.008Ba
	RE₂₅	0.160±0.005Bb	0.191±0.032Ba	0.174±0.008Bb
	RE₅₀	0.165±0.008Bb	0.171±0.006Ca	0.187±0.005Aa
	RE₇₅	0.237±0.008Aa	0.201±0.009Ab	0.233±0.019Aa

指标 Index	干旱胁迫处理 Drought stress treatment	氮添加水平 Nitrogen addition level	干旱胁迫处理×氮添加水平 Drought stress treatment × nitrogen addition level
总生物量Total biomass	65.66^**	88.93^**	13.52^**
净光合速率Net photosynthetic rate	146.60^**	43.24^**	14.78^**
水分利用效率Water use efficiency	69.98^**	29.79^**	15.65^**
导水率Hydraulic conductivity	71.07^**	232.60^**	65.70^**
可溶性糖Soluble sugars	83.06^**	154.50^**	11.35^**
淀粉Starch	5.24^**	152.30^**	5.67^**
非结构性碳水化合物Non-structural carbohydrates	42.68^**	491.00^**	6.43^**
脯氨酸Proline	5.89^**	231.50^**	20.56^**
K⁺	30.51^**	1.99^ns	5.75^**

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Osmotic Regulation Mechanism of Pinus tabuliformis Seedlings under the Joint Effects of Nitrogen Addition and Drought Stress

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