红皮云杉主要单萜烯对云杉八齿小蠹的毒力及行为调控作用

doi:10.11707/j.1001-7488.LYKX20220054

Abstract

Abstract:

Objective: In this study, we analyzed the differences in the release of monoterpenes from the trunk of healthy and weak Picea koraiensis, evaluated their fumigation toxicity to adult of Ips typographus, and tested their synergistic and antogonistic effects on aggregation pheromones of the bark beetle. Based on these investigation, we aims to produce efficient semiochemicals for population regulation of I. typographus in the forest. Method: Dynamic headspace sampling was used to collect the volatiles released from the stem of healthy and weak P. koraiensis. Gas chromatography was used to qualitatively and quantitatively analyze the monoterpenes. The toxicity of the monoterpenes against adult I. typographus was determined by using the headspace fumigation method, and the behavioral effects of the monoterpenes on I. typographus and its natural enemy, Thanasimus substriatus(Coleoptera: Cleridae) were analyzed in the forest. Result: The major monoterpenes from the trunk ofP. koraiensis were identified, including S-(–)-α-pinene, R-(+)-α-pinene, S-(–)-β-pinene, myrcene, 3-carene, (–)-limonene, and (+)-limonene. The total amount of monoterpenes in healthy stems was significantly higher than that in weakened stems, while the release amount of R-(+)-α-pinene and (+)-limonene showed no significant difference between healthy and weakened stems. The LC₅₀ values of seven monoterpenes against I. typographus ranged from 3.10–4.50 μL?mL^?1, with (+)-limonene showing the strongest toxicity. There were significant differences in LC₅₀ values among the seven monoterpenes, but not between male and female adult beetles. The field behavioral test showed that 3-carene, (–)-limonene, and (+)-limonene significantly reduced the trap catches of I. typographus by aggregation pheromones, but S-(–)-β-pinene showed no effect on the trap catches. Compared to (–)-limonene and (+)-limonene, 3-carene was able to significantly improve the trap catches of the predator T. substriatusby aggregation pheromones. Conclusion: The trunk of P. koraiensis can release seven major monoterpenes. The weakened spruce, suitable for colonization byI. typographus, releases less monoterpenes than the healthy spruce. These monoterpenes have fumigation toxicity against I. typographus and may be involved in regulating tritrophic relationships between spruce, I. typographus, and T. substriatus. Limonene can be used as a repellent for ecological prevention and control of I. typographus.

Key words: Ips typographus, Picea koraiensis, monoterpene, fumigation toxicity, behavioral regulation

CLC Number:

S763.3

Jiaxing Fang,Yue Wang,Mei Deng,Chunmei Yu,Fu Liu,Sufang Zhang,Zhen Zhang,Xiangbo Kong. Toxicity and Behavioral Regulatory Effects of Monoterpenes in Picea koraiensis to the Bark BeetleIps typographus (Coleoptera: Scolytidae)[J]. Scientia Silvae Sinicae, 2023, 59(4): 132-138.

Figures/Tables 5

Table 1

Fig.1

Table 2

Table 3

Fig.2

References 0

	程　彬, 张　健, 侯丽伟. 云杉八齿小蠹信息素的研究进展. 环境昆虫学报, 2018, 40 (2): 268- 275.
	Cheng B, Zhang J, Hou L W. Advances in the studies on semiochemicals in Ips typographus . Journal of Environmental Entomology, 2018, 40 (2): 268- 275.
	方加兴, 张苏芳, 刘　福, 等. 小蠹虫类异戊二烯类聚集信息素的生物合成. 昆虫学报, 2018, 61 (10): 1222- 1236. doi: 10.16380/j.kcxb.2018.10.012
	Fang J X, Zhang S F, Liu F, et al. Biosynthesis of isoprenoid aggregation pheromones in bark beetles (Coleoptera: Scolytidae). Acta Entomologica Sinica, 2018, 61 (10): 1222- 1236. doi: 10.16380/j.kcxb.2018.10.012
	孙晓玲. 2006. 云杉八齿小蠹化学信息物质的研究.哈尔滨: 东北林业大学.
	Sun X L. 2006. Study on semiochemicals of Ips typographus L. Harbin: Northeast Forestry University. ［in Chinese］
	萧刚柔. 1992. 中国森林昆虫. 第2版. 北京: 中国林业出版社.
	Xiao G R. 1992. Forest Insects of China. 2nd ed. Beijing: China Forestry Publishing House. ［in Chinese］
	Birgersson G, Bergström G. Volatiles released from individual spruce bark beetle entrance holes quantitative variations during the first week of attack. Journal of Chemical Ecology, 1989, 15 (10): 2465- 2483. doi: 10.1007/BF01020377
	Chiu C C, Keeling C I, Bohlmann J. Toxicity of pine monoterpenes to mountain pine beetle. Scientific Reports, 2017, 7, 8858. doi: 10.1038/s41598-017-08983-y
	Dicke M, Baldwin I T. The evolutionary context for herbivore-induced plant volatiles: beyond the “cry for help”. Trends in Plant Science, 2010, 15 (3): 167- 175. doi: 10.1016/j.tplants.2009.12.002
	Erbilgin N, Raffa K F. Modulation of predator attraction to pheromones of two prey species by stereochemistry of plant volatiles. Oecologia, 2001, 127 (3): 444- 453. doi: 10.1007/s004420000606
	Erbilgin N, Ma C, Whitehouse C, et al. Chemical similarity between historical and novel host plants promotes range and host expansion of the mountain pine beetle in a naive host ecosystem. New Phytologist, 2014, 201 (3): 940- 950. doi: 10.1111/nph.12573
	Fang J X, Zhang S F, Liu F, et al. Differences in gut bacterial communities of Ips typographus (Coleoptera: Curculionidae) induced by enantiomer-specific α-pinene . 2020. Environmental Entomology, 2020, 49 (5): 1198- 1205.
	Fang J X, Zhang S F, Liu F, et al. Functional investigation of monoterpenes for improved understanding of the relationship between hosts and bark beetles. Journal of Applied Entomology, 2021, 145 (4): 303- 311. doi: 10.1111/jen.12850
	Jakoby O, Lischke H, Wermelinger B. Climate change alters elevational phenology patterns of the European spruce bark beetle (Ips typographus) . Global Change Biology, 2019, 25 (12): 4048- 4063. doi: 10.1111/gcb.14766
	Koštál V, Doležal P, Rozsypal J, et al. Physiological and biochemical analysis of overwintering and cold tolerance in two Central European populations of the spruce bark beetle, Ips typographus . Journal of Insect Physiology, 2011, 57 (8): 1136- 1146. doi: 10.1016/j.jinsphys.2011.03.011
	Kurz W, Dymond C, Stinson G, et al. Mountain pine beetle and forest carbon feedback to climate change. Nature, 2008, 452 (7190): 987- 990. doi: 10.1038/nature06777
	Lei C, Sun X. Comparing lethal dose ratios using probit regression with arbitrary slopes. BMC Pharmacology and Toxicology, 2018, 19 (1): 61. doi: 10.1186/s40360-018-0250-1
	Mageroy M H, Christiansen E, Långström B, et al. Priming of inducible defenses protects Norway spruce against tree-killing bark beetles. Plant Cell and Environment, 2020, 43 (2): 420- 430. doi: 10.1111/pce.13661
	Mayer F, Piel F B, Cassel-Lundhagen A, et al. Comparative multilocus phylogeography of two Palaearctic spruce bark beetles: influence of contrasting ecological strategies on genetic variation. Molecular Ecology, 2015, 24 (6): 1292- 1310. doi: 10.1111/mec.13104
	Netherer S, Kandasamy D, Jirosová A, et al. Interactions among Norway spruce, the bark beetle Ips typographus and its fungal symbionts in times of drought . Journal of Pest Science, 2021, 94 (3): 591- 614. doi: 10.1007/s10340-021-01341-y
	Seybold S J, Bentz B J, Fettig C J, et al. Management of western North American bark beetles with semiochemicals. Annual Review of Entomology, 2018, 63 (1): 407- 432. doi: 10.1146/annurev-ento-020117-043339
	Seybold S J, Huber D P W, Lee J F, et al. Pine monoterpenes and pine bark beetles: a marriage of convenience for defense and chemical communication. Phytochemistry Reviews, 2006, 5 (1): 143- 178. doi: 10.1007/s11101-006-9002-8
	Smith R H. Effect of monoterpene vapors on the western pine beetle. Journal of Economic Entomology, 1965, 58 (3): 509- 511. doi: 10.1093/jee/58.3.509
	Tittiger C, Blomquist G J. Pheromone biosynthesis in bark beetles. Current Opinion in Insect Science, 2017, 24, 68- 74. doi: 10.1016/j.cois.2017.09.005
	Turlings T C J, Erb M. Tritrophic interactions mediated by herbivore-induced plant volatiles: mechanisms, ecological relevance, and application potential. Annual Review of Entomology, 2018, 63 (1): 433- 452. doi: 10.1146/annurev-ento-020117-043507
	Werner R A. Toxicity and repellency of 4-allylanisole and monoterpenes from white spruce and tamarack to the spruce beetle and eastern larch beetle (Coleoptera: Scolytidae). Environmental Entomology, 1995, 24 (2): 372- 379. doi: 10.1093/ee/24.2.372
	Zhang Q H, Song L W, Ma J H, et al. Aggregation pheromone of a newly described spruce bark beetle, Ips shangrila Cognato and Sun from China . Chemoecology, 2009, 19 (4): 203- 210. doi: 10.1007/s00049-009-0026-6

试验处理 Treatment	诱芯配方及含量 Lure formula and contents
试验处理 Treatment	2-甲基-3-丁烯- 2-醇 2-methyl-3- buten-2-ol	S-(–)-顺式-马鞭草烯醇S-(–)-cis-verbenol	萜烯成分和含量 Monoterpenes and contents
正对照 Positive control	5 mL	40 mg	—
处理1 Treatment 1	5 mL	40 mg	S-(–)-β-蒎烯（4 mL）
处理2 Treatment 2	5 mL	40 mg	3-蒈烯（4 mL）
处理3 Treatment 3	5 mL	40 mg	(–)-柠檬烯（4 mL）
处理4 Treatment 4	5 mL	40 mg	(+)-柠檬烯（4 mL）
空白对照 Blank control	—	—	—

峰 Peak	单萜烯 Monoterpenes	保留时间 Retention time/ min	健康红皮云杉 Healthy tree/ (μg·h^?1)	衰弱红皮云杉 Weakened tree/ (μg·h^?1)	统计学参数 Statistical parameters
1	S-(–)-α-蒎烯 S-(–)-α-pinene	17.210	277.40±32.22a	144.64±27.85b	t=3.11,P=0.03
2	R-(+)-α-蒎烯 R-(+)-α-pinene	17.476	52.12±20.40a	20.46±6.90a	t=1.47,P=0.22
3	月桂烯 Myrcene	18.388	91.80±11.55a	39.85±6.25b	t=3.95,P=0.01
4	S-(–)-β-蒎烯 S-(–)-β-pinene	19.417	548.15±83.81a	281.25±27.86b	t=3.02,P=0.03
5	3-蒈烯 3-carene	19.719	180.33±15.82a	86.41±24.42b	t=3.25,P=0.03
6	(–)-柠檬烯 (–)-limonene	20.703	22.08±6.89a	4.33±1.17b	t=4.15,P=0.01
7	(+)-柠檬烯 (+)-limonene	21.150	168.21±48.98a	118.51±10.90a	t=0.99, P=0.38
单萜烯总含量 Total amount of monoterpenes			1340.10±137.08a	695.46±55.24b	t=4.36,P=0.01

单萜烯 Monoterpene	致死中浓度（顶空熏蒸法） LC₅₀ (data obtained from the headspace exposure fumigation method)
单萜烯 Monoterpene	雄成虫Male LC₅₀ (CI 95%)	雌成虫Female LC₅₀ (CI 95%)
S-(–)-α-蒎烯 S-(–)-α-pinene	3.44 (3.10~3.82) bc	3.49 (1.82~5.36) bc
R-(+)-α-蒎烯 R-(+)-α-pinene	4.08 (2.94~5.64) ab	3.59 (3.24~3.94) ab
月桂烯 Myrcene	4.36 (3.16~6.22) a	4.11 (3.11~5.27) a
S-(–)-β-蒎烯 S-(–)-β-pinene	4.40 (3.21~6.13) a	3.72 (2.75~4.79) ab
3-蒈烯 3-carene	4.27 (3.83~4.77) a	3.67 (2.49~5.02) ab
(–)-柠檬烯 (–)-limonene	3.82 (2.48~5.47) bc	3.81 (2.51~5.31) ab
(+)-柠檬烯 (+)-limonene	3.23 (2.87~3.58) c	3.13 (2.74~3.51) c

[1]	Wang Lixiang, Liu Xiaobo, Ren Lili, Shi Juan, Luo Youqing. Variety of Endophytic Fungi Associated with Conifers in Mixed Conifer Forests Invaded by Sirex noctilio [J]. Scientia Silvae Sinicae, 2017, 53(9): 81-89.
[2]	Hua Shengzhou, Chen Jungang, Yu Xinxiao, Bi Huaxing, Fu Yanlin, Chen Jing, Sun Fengbin. Correlation between Terpenes Emission from Typical Forest Tree Species and Environmental Elements in Temperate Zone [J]. Scientia Silvae Sinicae, 2016, 52(11): 19-28.
[3]	Chen Dafeng, Li Yejing, Kong Xiangbo, Zhang Zhen, Wang Hongbin, Jiao Xiangjie, Duan Ruilong, Chen Jie, Li Jifeng. Olfactory Reception of Conspecific Aggregation Pheromone and Host Volatile Terpenes by Asian Larch Bark Beetle, Ips subelongatus [J]. Scientia Silvae Sinicae, 2013, 49(11): 89-97.
[4]	Chen Haibo;Zhang Zhen;Wang Hongbin;Kong Xiangbo;Liu Suicun. Variance in Relative Concentration of Induced Volatile Monoterpenes Composition of Pinus tabulaeformis by Dendroctonus valens [J]. Scientia Silvae Sinicae, 2012, 48(1): 97-102.
[5]	Tang Li;Tang Fang;Duan Jinghua;Liu Youquan;Zhong Qiuping .. Cloning and Sequence Analysis of a Homologous Linalool Synthase Gene Involved in Floral Scents in Osmanthus fragrans var. thunbergii [J]. Scientia Silvae Sinicae, 2009, 12(5): 11-19.
[6]	Zeng Yihui;Hu Xianju. GEOGRAPHIC VARIATION IN COMPONENTS OF MONOTERPENE AND SESQUITERPENE IN PINUS ARMANDI [J]. , 1992, 28(2): 161-166.
[7]	Sun Liyan;Zou Yinlian;Yuan Dajin. ANALYSIS OF CHEMICAL CONSTITUENTS OF ESSENTIAL OIL FROM PICEA ASPERATA MAST [J]. , 1991, 27(3): 289-291.

Toxicity and Behavioral Regulatory Effects of Monoterpenes in Picea koraiensis to the Bark BeetleIps typographus (Coleoptera: Scolytidae)

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 0

Related Articles 7

Recommended Articles

Metrics

Comments