落叶松八齿小蠹对聚集信息素和寄主挥发性萜烯成分的电生理响应

doi:10.11707/j.1001-7488.20131112

Abstract

Abstract:

Terpenes volatiles from the stem barks and needles of Dahurian larch (Larix gmelinii), Prince Rupprecht larch (Larix principis rupprechtii), and Korean larch (Larix olgensis), which are common hosts in the range of Ips subelongatus in northeastern China, were entrapped by dynamic headspace collection technique, and then the hexane elution of the volatiles was analyzed by chiral gas chromatography (GC) and GC-mass spectrometry (MS) in order to identify components of the volatiles. Totally 12 monoterpenes was identified, which are mainly consists of S-(-)-α-pinene, R-(+)-α-pinene, 3-carene, R-(+)-β-pinene, and S-(-)-β-pinene accounting for more than 80% of the total collected monoterpenes. Furthermore, the olfactory sensitivity and selectivity of I. subelongatus to synthetic host volatiles were assayed by a coupled gas chromatograph-electroantennographic detector (GC-EAD) and electroantennogram (EAG). The olfactory responsiveness to S-(-)-α-pinene, 3-carene, (+)-limonene, γ-terpinene, and terpinolene was assessed in GC-EAD analyses. In addition, p-cymene also elicited a strong EAD response, but it was not detected from the headspace volatile of healthy trees in the chiral GC analyses. As for the aggregation pheromone components, the strongest EAD response was obtained with (-)-ipsenol on both female and male antennae, however, different EAD response patterns to (+)-and (-)-ipsdienol on male and female antennae were observed in the GC-EAD analyses. The thresholds of EAG responsiveness to synthetic monoterpenes and aggregation pheromones were 0.1 μg, but the saturation levels of different compounds were different. Finally, we discussed the relationship of host volatiles and aggregation pheromone of bark beetles.

Key words: larch, Ips subelongatus, aggregation pheromone, monoterpenes, sensitivity, EAG, GC-EAD

CLC Number:

S718.7

Chen Dafeng, Li Yejing, Kong Xiangbo, Zhang Zhen, Wang Hongbin, Jiao Xiangjie, Duan Ruilong, Chen Jie, Li Jifeng. Olfactory Reception of Conspecific Aggregation Pheromone and Host Volatile Terpenes by Asian Larch Bark Beetle, Ips subelongatus[J]. Scientia Silvae Sinicae, 2013, 49(11): 89-97.

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Olfactory Reception of Conspecific Aggregation Pheromone and Host Volatile Terpenes by Asian Larch Bark Beetle, Ips subelongatus

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